Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 3300332..3300859 Replicon chromosome
Accession NZ_CP050242
Organism Erwinia amylovora strain FB-307

Toxin (Protein)


Gene name relE Uniprot ID D4HV72
Locus tag EHW99_RS14935 Protein ID WP_004155192.1
Coordinates 3300332..3300607 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID D4HV71
Locus tag EHW99_RS14940 Protein ID WP_004155191.1
Coordinates 3300611..3300859 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHW99_RS14920 3296051..3299416 + 3366 WP_004155194.1 ATP-binding protein -
EHW99_RS14925 3299565..3299717 - 153 WP_004163933.1 hypothetical protein -
EHW99_RS14930 3299713..3299913 - 201 Protein_2875 transposase -
EHW99_RS14935 3300332..3300607 - 276 WP_004155192.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EHW99_RS14940 3300611..3300859 - 249 WP_004155191.1 ribbon-helix-helix domain-containing protein Antitoxin
EHW99_RS14945 3300956..3301789 - 834 WP_004155190.1 DUF4942 domain-containing protein -
EHW99_RS14950 3301902..3302222 - 321 WP_004155189.1 TA system toxin CbtA family protein -
EHW99_RS14955 3302259..3302582 - 324 WP_004155188.1 type IV toxin-antitoxin system YeeU family antitoxin -
EHW99_RS14960 3302595..3303065 - 471 WP_004155187.1 DNA repair protein RadC -
EHW99_RS14965 3303136..3303710 - 575 Protein_2882 DUF932 domain-containing protein -
EHW99_RS14970 3303935..3304177 - 243 WP_004155184.1 hypothetical protein -
EHW99_RS14975 3305387..3305584 + 198 WP_004155182.1 AlpA family phage regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3300332..3323509 23177
flank IS/Tn - - 3299689..3299913 224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-43)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 11108.44 Da        Isoelectric Point: 5.5782

>T152408 WP_004155192.1 NZ_CP050242:c3300607-3300332 [Erwinia amylovora]
MEIQWTQKAQDDLERIYRFACQYSRQHADNVLERLLIGTSGLADHPRIGVSQIRYEPREVRKILFDDYDVHYEIQRDTIY
IVDLWHTREDR

Download         Length: 276 bp

>T152408 NZ_CP066144:2833342-2833449 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCGTGATTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTTGGCTGGTGGCGTAGCCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9121.53 Da        Isoelectric Point: 8.7560

>AT152408 WP_004155191.1 NZ_CP050242:c3300859-3300611 [Erwinia amylovora]
MRQLAKQTISAQIPAELAAAVENLAIELDRSKSWVIKEALTAMIEERERRHQQILKGLADVDAGRVVSHADVVDFANKLK
KS

Download         Length: 249 bp

>AT152408 NZ_CP066144:c2833286-2833225 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTGACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A831EQZ9

Antitoxin

Source ID Structure
AlphaFold DB A0A831A0W7

References