Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1621872..1622552 Replicon chromosome
Accession NZ_CP076595
Organism Erwinia amylovora isolate CP20130202

Toxin (Protein)


Gene name yeeV Uniprot ID D4HV69
Locus tag KQH34_RS07565 Protein ID WP_004155189.1
Coordinates 1621872..1622192 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D4HV68
Locus tag KQH34_RS07570 Protein ID WP_004155188.1
Coordinates 1622229..1622552 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH34_RS07545 (KQH34_07530) 1619683..1619883 - 201 Protein_1464 transposase -
KQH34_RS07550 (KQH34_07535) 1620302..1620577 - 276 WP_004155192.1 type II toxin-antitoxin system RelE/ParE family toxin -
KQH34_RS07555 (KQH34_07540) 1620581..1620829 - 249 WP_004155191.1 ribbon-helix-helix domain-containing protein -
KQH34_RS07560 (KQH34_07545) 1620926..1621759 - 834 WP_004155190.1 DUF4942 domain-containing protein -
KQH34_RS07565 (KQH34_07550) 1621872..1622192 - 321 WP_004155189.1 TA system toxin CbtA family protein Toxin
KQH34_RS07570 (KQH34_07555) 1622229..1622552 - 324 WP_004155188.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KQH34_RS07575 (KQH34_07560) 1622565..1623035 - 471 WP_004155187.1 DNA repair protein RadC -
KQH34_RS07580 (KQH34_07565) 1623106..1623680 - 575 Protein_1471 DUF932 domain-containing protein -
KQH34_RS07585 (KQH34_07570) 1623905..1624147 - 243 WP_004155184.1 hypothetical protein -
KQH34_RS07590 (KQH34_07575) 1625357..1625554 + 198 WP_004155182.1 AlpA family phage regulatory protein -
KQH34_RS07595 1625662..1626012 + 351 WP_004155181.1 hypothetical protein -
KQH34_RS07600 (KQH34_07585) 1626456..1627541 + 1086 WP_004155179.1 DNA sulfur modification protein DndB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1620302..1643473 23171
flank IS/Tn - - 1619659..1619883 224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(5-105)

Antitoxin

Antitoxin CbeA

(7-106)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12023.69 Da        Isoelectric Point: 5.4732

>T206017 WP_004155189.1 NZ_CP076595:c1622192-1621872 [Erwinia amylovora]
MHISTVPATVPVSSRLSPVQVWQQLLTYLLEHHYGLTLNDTPFHDDAAIEEHIDAGITLADAVNFLVERYELVRIDRKGF
TWQEQTPFLAATDILRARRATGLINI

Download         Length: 321 bp

>T206017 NZ_CP102068:18030-18893 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAACTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTACAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATAGCAGTACCGAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCAAAAATACCTAAGCTTCCAGGACTTTAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12088.81 Da        Isoelectric Point: 6.4689

>AT206017 WP_004155188.1 NZ_CP076595:c1622552-1622229 [Erwinia amylovora]
MSITLPHEWGLQSDITPRFGARLVQEGNRLYYLADRAGLTVSFSPIHLQKLDQAFPLFVEQLENMLRAGELNPHQQHQVT
IQLTSLTCIADTRGSCGYVYISIYPTP

Download         Length: 324 bp

>AT206017 NZ_CP102068:17756-18028 [Enterococcus faecalis]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A831EPU6

Antitoxin

Source ID Structure
AlphaFold DB A0A831ENV6

References