Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3304712..3305392 Replicon chromosome
Accession NZ_CP024970
Organism Erwinia amylovora strain E-2

Toxin (Protein)


Gene name yeeV Uniprot ID D4HV69
Locus tag AD997_RS15465 Protein ID WP_004155189.1
Coordinates 3304712..3305032 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D4HV68
Locus tag AD997_RS15470 Protein ID WP_004155188.1
Coordinates 3305069..3305392 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AD997_RS18190 3302375..3302527 - 153 WP_004163933.1 hypothetical protein -
AD997_RS15445 3302523..3302723 - 201 Protein_2874 transposase -
AD997_RS15450 3303142..3303417 - 276 WP_004155192.1 type II toxin-antitoxin system RelE/ParE family toxin -
AD997_RS15455 3303421..3303669 - 249 WP_004155191.1 ribbon-helix-helix domain-containing protein -
AD997_RS15460 3303766..3304599 - 834 WP_004155190.1 DUF4942 domain-containing protein -
AD997_RS15465 3304712..3305032 - 321 WP_004155189.1 TA system toxin CbtA family protein Toxin
AD997_RS15470 3305069..3305392 - 324 WP_004155188.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
AD997_RS15475 3305405..3305875 - 471 WP_004155187.1 DNA repair protein RadC -
AD997_RS15480 3305946..3306520 - 575 Protein_2881 DUF932 domain-containing protein -
AD997_RS15485 3306745..3306987 - 243 WP_004155184.1 hypothetical protein -
AD997_RS15495 3308197..3308394 + 198 WP_004155182.1 AlpA family phage regulatory protein -
AD997_RS18450 3308502..3309096 + 595 Protein_2884 hypothetical protein -
AD997_RS15505 3309296..3310381 + 1086 WP_004155179.1 DNA sulfur modification protein DndB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3303142..3326289 23147
flank IS/Tn - - 3302499..3302723 224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(5-105)

Antitoxin

Antitoxin CbeA

(7-106)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12023.69 Da        Isoelectric Point: 5.4732

>T89364 WP_004155189.1 NZ_CP024970:c3305032-3304712 [Erwinia amylovora]
MHISTVPATVPVSSRLSPVQVWQQLLTYLLEHHYGLTLNDTPFHDDAAIEEHIDAGITLADAVNFLVERYELVRIDRKGF
TWQEQTPFLAATDILRARRATGLINI

Download         Length: 321 bp

>T89364 NZ_CP024970:c3305032-3304712 [Erwinia amylovora]
ATGCACATTTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCTCCCGTGCAGGTGTGGCAGCAACTTTTAAC
GTATCTGCTGGAGCATCATTACGGCTTGACACTAAATGACACGCCATTCCATGACGACGCGGCCATTGAGGAGCATATCG
ACGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGTATCGACCGCAAAGGATTT
ACATGGCAGGAGCAGACGCCATTCCTGGCCGCCACCGATATTCTCAGAGCAAGGCGAGCTACCGGATTAATAAATATTTA
A

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12088.81 Da        Isoelectric Point: 6.4689

>AT89364 WP_004155188.1 NZ_CP024970:c3305392-3305069 [Erwinia amylovora]
MSITLPHEWGLQSDITPRFGARLVQEGNRLYYLADRAGLTVSFSPIHLQKLDQAFPLFVEQLENMLRAGELNPHQQHQVT
IQLTSLTCIADTRGSCGYVYISIYPTP

Download         Length: 324 bp

>AT89364 NZ_CP024970:c3305392-3305069 [Erwinia amylovora]
ATGTCAATCACACTACCTCATGAATGGGGTCTACAAAGCGATATCACCCCACGATTTGGCGCGAGATTAGTTCAGGAAGG
CAATCGGCTGTATTACCTGGCTGACAGGGCTGGATTAACGGTGAGCTTTAGCCCGATACACCTGCAGAAACTGGATCAGG
CTTTCCCGCTGTTTGTAGAACAACTGGAAAATATGCTGCGTGCTGGCGAACTAAATCCACATCAGCAACATCAGGTGACC
ATTCAACTTACGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGCGGTTACGTTTACATTTCTATTTATCCAACCCC
ATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A831EPU6

Antitoxin

Source ID Structure
AlphaFold DB A0A831ENV6

References