Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2001426..2002106 Replicon chromosome
Accession NZ_CP076591
Organism Erwinia amylovora isolate CP201142

Toxin (Protein)


Gene name yeeV Uniprot ID D4HV69
Locus tag KQH37_RS09190 Protein ID WP_004155189.1
Coordinates 2001426..2001746 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D4HV68
Locus tag KQH37_RS09195 Protein ID WP_004155188.1
Coordinates 2001783..2002106 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQH37_RS09170 (KQH37_09175) 1999237..1999437 - 201 Protein_1783 transposase -
KQH37_RS09175 (KQH37_09180) 1999856..2000131 - 276 WP_004155192.1 type II toxin-antitoxin system RelE/ParE family toxin -
KQH37_RS09180 (KQH37_09185) 2000135..2000383 - 249 WP_004155191.1 ribbon-helix-helix domain-containing protein -
KQH37_RS09185 (KQH37_09190) 2000480..2001313 - 834 WP_004155190.1 DUF4942 domain-containing protein -
KQH37_RS09190 (KQH37_09195) 2001426..2001746 - 321 WP_004155189.1 TA system toxin CbtA family protein Toxin
KQH37_RS09195 (KQH37_09200) 2001783..2002106 - 324 WP_004155188.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KQH37_RS09200 (KQH37_09205) 2002119..2002589 - 471 WP_004155187.1 DNA repair protein RadC -
KQH37_RS09205 (KQH37_09210) 2002660..2003234 - 575 Protein_1790 DUF932 domain-containing protein -
KQH37_RS09210 (KQH37_09215) 2003459..2003701 - 243 WP_004155184.1 hypothetical protein -
KQH37_RS09215 (KQH37_09220) 2004911..2005108 + 198 WP_004155182.1 AlpA family phage regulatory protein -
KQH37_RS09220 2005216..2005566 + 351 WP_004155181.1 hypothetical protein -
KQH37_RS09225 (KQH37_09230) 2006010..2007095 + 1086 WP_004155179.1 DNA sulfur modification protein DndB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1999856..2023027 23171
flank IS/Tn - - 1999213..1999437 224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(5-105)

Antitoxin

Antitoxin CbeA

(7-106)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12023.69 Da        Isoelectric Point: 5.4732

>T205987 WP_004155189.1 NZ_CP076591:c2001746-2001426 [Erwinia amylovora]
MHISTVPATVPVSSRLSPVQVWQQLLTYLLEHHYGLTLNDTPFHDDAAIEEHIDAGITLADAVNFLVERYELVRIDRKGF
TWQEQTPFLAATDILRARRATGLINI

Download         Length: 321 bp

>T205987 NZ_CP102061:c515777-515675 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12088.81 Da        Isoelectric Point: 6.4689

>AT205987 WP_004155188.1 NZ_CP076591:c2002106-2001783 [Erwinia amylovora]
MSITLPHEWGLQSDITPRFGARLVQEGNRLYYLADRAGLTVSFSPIHLQKLDQAFPLFVEQLENMLRAGELNPHQQHQVT
IQLTSLTCIADTRGSCGYVYISIYPTP

Download         Length: 324 bp

>AT205987 NZ_CP102061:515637-515905 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A831EPU6

Antitoxin

Source ID Structure
AlphaFold DB A0A831ENV6

References