6360

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type I Classification (family/domain) sprG-sprF/-
Location 2010870..2011135 Replicon chromosome
Accession NC_002745
Organism Staphylococcus aureus subsp. aureus N315
T1TAdb ID TA03804

Toxin (Protein)


Gene name SprG Uniprot ID Q2FWU9
Locus tag SAS059 Protein ID WP_011447039.1
Coordinates 2011001..2011135 (-) Length 45 a.a.

Antitoxin (RNA)


Gene name SprF
Locus tag SprF
Coordinates 2010870..2011011 (+)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SA_RS10080 2006215..2006475 + 261 WP_001791826.1 hypothetical protein -
SA_RS10085 (SA1754) 2006528..2006878 - 351 WP_000702262.1 complement inhibitor SCIN-A -
SA_RS10090 (SA1755) 2007561..2008010 + 450 WP_000727645.1 chemotaxis-inhibiting protein CHIPS -
SA_RS10095 2008105..2008439 - 335 Protein_1877 SH3 domain-containing protein -
SA_RS10100 (SA1758) 2009086..2009577 - 492 WP_000920042.1 staphylokinase -
SA_RS10105 (SA1759) 2009768..2010523 - 756 WP_000861026.1 CHAP domain-containing protein -
SA_RS10110 (SA1760) 2010535..2010789 - 255 WP_000611512.1 phage holin -
- 2010870..2011011 + 142 - - Antitoxin
- 2011001..2011135 - 135 - - Toxin
SA_RS10125 (SA1761) 2011380..2012153 - 774 WP_025174055.1 staphylococcal enterotoxin type P -
SA_RS10130 (SA1762) 2012574..2012948 - 375 WP_000340977.1 hypothetical protein -
SA_RS10135 (SA1763) 2013004..2013291 - 288 WP_001262621.1 hypothetical protein -
SA_RS10140 (SAS061) 2013337..2013489 - 153 WP_001000058.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - scn / chp / sak / see / hlb / sell / sec / tsst-1 / groEL 2006528..2074317 67789


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Small toxic protein BsrE-like

(16-44)


Sequences

Toxin        


Download         Length: 45 a.a.        Molecular weight: 5029.35 Da        Isoelectric Point: 11.7213

>T6360 WP_011447039.1 NC_002745:c2011135-2011001 [Staphylococcus aureus subsp. aureus N315]
MVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Download         Length: 135 bp

>T6360 NC_002745:c2011135-2011001 [Staphylococcus aureus subsp. aureus N315]
ATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCTAATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCT
TATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAATTAAGCAATAAAAAATAA

Antitoxin


Download         Length: 142 bp

>AT6360 NC_002745:2010870-2011011 [Staphylococcus aureus subsp. aureus N315]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7NS1

Antitoxin

Download structure file

References

(1) Marie-Laure Pinel-Marie et al. (2014) Dual toxic-peptide-coding Staphylococcus aureus RNA under antisense regulation targets host cells and bacterial rivals unequally. Cell Reports 7(2):424-435. [PubMed:24703849] experimental

(2) Marie-Laure Pinel-Marie et al. (2021) RNA antitoxin SprF1 binds ribosomes to attenuate translation and promote persister cell formation in Staphylococcus aureus. Nature Microbiology 6(2):209-220. [PubMed:33398097] experimental

(3) Camille Riffaud et al. (2019) Functionality and cross-regulation of the four SprG/SprF type I toxin-antitoxin systems in Staphylococcus aureus. Nucleic Acids Research 47(4):1740-1758. [PubMed:30551143] experimental


experimental experimental literature