Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type I Classification (family/domain) sprG-sprF/-
Location 34711..35007 Replicon chromosome
Accession NZ_JPPU01000005
Organism Staphylococcus aureus strain HG003 isolate RN1 Scaffold_5

Toxin (Protein)


Gene name SprG1 Uniprot ID -
Locus tag - Protein ID -
Coordinates 34711..34842 (+) Length 44 a.a.

Antitoxin (RNA)


Gene name SprF1
Locus tag -
Coordinates 34833..35007 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM26_RS18550 (IM26_03465) 29904..33689 + 3786 WP_000582137.1 phage tail spike protein -
IM26_RS0113950 33679..33831 + 153 WP_001153681.1 hypothetical protein -
IM26_RS18555 (IM26_03475) 33878..34165 + 288 WP_001040261.1 hypothetical protein -
IM26_RS0113955 (IM26_03480) 34223..34519 + 297 WP_000539688.1 DUF2951 domain-containing protein -
- 34711..34842 + 132 - - Toxin
- 34833..35007 - 175 - - Antitoxin
IM26_RS18560 (IM26_03490) 35057..35311 + 255 WP_000611512.1 phage holin -
IM26_RS18565 (IM26_03495) 35323..36078 + 756 WP_000861038.1 CHAP domain-containing protein -
IM26_RS18570 (IM26_03500) 36269..36760 + 492 WP_000920037.1 staphylokinase -
IM26_RS29050 (IM26_03505) 37410..37745 + 336 Protein_62 SH3 domain-containing protein -
IM26_RS18580 (IM26_03510) 37840..38289 - 450 WP_000727643.1 chemotaxis-inhibiting protein CHIPS -
IM26_RS18585 (IM26_03515) 38972..39322 + 351 WP_000702262.1 complement inhibitor SCIN-A -
IM26_RS0113965 39375..39635 - 261 WP_001791826.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - sak / chp / scn 180..39322 39142


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Small toxic protein BsrE-like

(16-44)


Sequences

Toxin        


Download         Length: 44 a.a.        Molecular weight: 5029.35 Da        Isoelectric Point: 11.7213

>T10146 - NZ_JPPU01000005:34711-34842 [Staphylococcus aureus]
MVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Download         Length: 132 bp

>T10146 NZ_JPPU01000005:34711-34842 [Staphylococcus aureus]
ATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCTAATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCT
TATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAATTAAGCAATAAAAAA

Antitoxin


Download         Length: 175 bp

>AT10146 NZ_JPPU01000005:c35007-34833 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Download structure file

References

(1) Camille Riffaud et al. (2019) Functionality and cross-regulation of the four SprG/SprF type I toxin-antitoxin systems in Staphylococcus aureus. Nucleic Acids Research 47(4):1740-1758. [PubMed:30551143] experimental


experimental experimental literature