Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | txpA-ratA/- |
| Location | 13585..13855 | Replicon | plasmid pTEF1 |
| Accession | NC_004669 | ||
| Organism | Enterococcus faecalis V583 | ||
| T1TAdb ID | TA05742 | ||
Toxin (Protein)
| Gene name | txpA | Uniprot ID | - |
| Locus tag | EF_RS16890 | Protein ID | WP_228770506.1 |
| Coordinates | 13585..13665 (+) | Length | 27 a.a. |
Antitoxin (RNA)
| Gene name | ratA | ||
| Locus tag | - | ||
| Coordinates | 13599..13855 (-) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EF_RS16210 (EF_A0010) | 8712..9035 | + | 324 | WP_010730188.1 | quaternary ammonium compound efflux SMR transporter QacH | - |
| EF_RS16215 (EF_A0012) | 9848..10771 | - | 924 | WP_025192524.1 | protein rep | - |
| EF_RS16220 (EF_A0013) | 10846..11526 | - | 681 | WP_001015311.1 | IS6-like element IS1216 family transposase | - |
| EF_RS16225 (EF_A0014) | 11564..12055 | - | 492 | WP_010774254.1 | ABC transporter transmembrane domain-containing protein | - |
| EF_RS16230 (EF_A0015) | 12099..12233 | - | 135 | WP_010708487.1 | LsbB family leaderless bacteriocin | - |
| EF_RS16235 (EF_A0016) | 12615..13304 | - | 690 | WP_002387615.1 | IS6 family transposase | - |
| EF_RS16890 | 13585..13665 | + | 81 | WP_228770506.1 | putative holin-like toxin | Toxin |
| - | 13599..13855 | - | 257 | - | - | Antitoxin |
| EF_RS16245 (EF_A0019) | 13884..14360 | - | 477 | WP_000615486.1 | single-stranded DNA-binding protein | - |
| EF_RS16250 (EF_A0020) | 14500..15816 | - | 1317 | Protein_17 | PcfJ domain-containing protein | - |
| EF_RS16255 (EF_A0021) | 15830..16585 | - | 756 | WP_002387613.1 | Cas9 inhibitor AcrIIA9 family protein | - |
| EF_RS16260 (EF_A0022) | 16747..17013 | - | 267 | WP_001245899.1 | hypothetical protein | - |
| EF_RS16265 | 17020..17202 | - | 183 | WP_001808764.1 | hypothetical protein | - |
| EF_RS16270 (EF_A0024) | 17620..18108 | - | 489 | WP_010708485.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Non-Mobilizable plasmid | erm(B) / qacZ / aac(6')-aph(2'') | asa1 | 1..66320 | 66320 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 27 a.a. Molecular weight: 2900.56 Da Isoelectric Point: 10.4801
>T10207 WP_228770506.1 NC_004669:13585-13665 [Enterococcus faecalis V583]
MLAFGGFTLTLITTIVAILNYKDKKK
MLAFGGFTLTLITTIVAILNYKDKKK
Download Length: 81 bp
>T10207 NC_004669:13585-13665 [Enterococcus faecalis V583]
ATGCTTGCGTTTGGTGGGTTTACATTGACCCTAATCACAACCATTGTTGCCATTCTGAACTATAAAGACAAAAAGAAATA
A
ATGCTTGCGTTTGGTGGGTTTACATTGACCCTAATCACAACCATTGTTGCCATTCTGAACTATAAAGACAAAAAGAAATA
A
Antitoxin
Download Length: 257 bp
>AT10207 NC_004669:c13855-13599 [Enterococcus faecalis V583]
CACCGCCACCGTCGTTTAGGCGCGCATGAAAAAAGGTAGGCATTCGCCTACCTTTTCGCACAAAATGTGGTATAATAATT
ATGTAAAGAGGACGTACTGGTAATACGCCCTCATTGATAGCACCGCTAAAGACGGTGGCAGAGTTTTTATAAGTTATTTA
GAAATAACCGGTCTCCTGCTAAAGTGACGGTTATTTCTTTTTGTCTTTATAGTTCAGAATGGCAACAATGGTTGTGATTA
GGGTCAATGTAAACCCA
CACCGCCACCGTCGTTTAGGCGCGCATGAAAAAAGGTAGGCATTCGCCTACCTTTTCGCACAAAATGTGGTATAATAATT
ATGTAAAGAGGACGTACTGGTAATACGCCCTCATTGATAGCACCGCTAAAGACGGTGGCAGAGTTTTTATAAGTTATTTA
GAAATAACCGGTCTCCTGCTAAAGTGACGGTTATTTCTTTTTGTCTTTATAGTTCAGAATGGCAACAATGGTTGTGATTA
GGGTCAATGTAAACCCA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10204 | Enterococcus faecalis V583 |
53.846 |
100 |
0.538 |
| T10025 | Staphylococcus aureus subsp. aureus N315 |
52 |
100 |
0.52 |
| T10049 | Staphylococcus aureus strain HG003 isolate RN1 Scaffold_9 |
52 |
96.154 |
0.5 |
| T10205 | Enterococcus faecalis V583 |
46.154 |
100 |
0.462 |
| T6360 | Staphylococcus aureus subsp. aureus N315 |
36 |
96.154 |
0.346 |
| T10146 | Staphylococcus aureus strain HG003 isolate RN1 Scaffold_5 |
36 |
96.154 |
0.346 |
| T10154 | Staphylococcus aureus subsp. aureus MW2 |
36 |
96.154 |
0.346 |
| T10201 | Bacillus subtilis subsp. subtilis str. 168 |
60 |
57.692 |
0.346 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Elizabeth M Fozo et al. (2010) Abundance of type I toxin-antitoxin systems in bacteria: searches for new candidates and discovery of novel families. Nucleic Acids Research 38(11):3743-59. [PubMed:20156992]