Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | higBA (relBE)/HTH(antitoxin) |
Location | 4504881..4505483 | Replicon | chromosome |
Accession | NZ_CP007799 | ||
Organism | Escherichia coli Nissle 1917 |
Toxin (Protein)
Gene name | higB | Uniprot ID | S1P416 |
Locus tag | ECOLIN_RS22410 | Protein ID | WP_000897302.1 |
Coordinates | 4504881..4505192 (+) | Length | 104 a.a. |
Antitoxin (Protein)
Gene name | higA | Uniprot ID | - |
Locus tag | ECOLIN_RS22415 | Protein ID | WP_000356397.1 |
Coordinates | 4505193..4505483 (+) | Length | 97 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
ECOLIN_RS22385 (ECOLIN_21650) | 4500363..4500800 | + | 438 | WP_000560983.1 | D-tyrosyl-tRNA(Tyr) deacylase | - |
ECOLIN_RS22390 (ECOLIN_21655) | 4500845..4501786 | + | 942 | WP_024199419.1 | fatty acid biosynthesis protein FabY | - |
ECOLIN_RS22395 (ECOLIN_21660) | 4501850..4502758 | - | 909 | WP_001305059.1 | alpha/beta hydrolase | - |
ECOLIN_RS22400 (ECOLIN_21665) | 4502887..4503405 | + | 519 | WP_000115885.1 | ClbS/DfsB family four-helix bundle protein | - |
ECOLIN_RS22405 (ECOLIN_21670) | 4503424..4504644 | + | 1221 | WP_000343765.1 | ISL3-like element ISEc53 family transposase | - |
ECOLIN_RS22410 (ECOLIN_21675) | 4504881..4505192 | + | 312 | WP_000897302.1 | hypothetical protein | Toxin |
ECOLIN_RS22415 (ECOLIN_21680) | 4505193..4505483 | + | 291 | WP_000356397.1 | helix-turn-helix domain-containing protein | Antitoxin |
ECOLIN_RS22425 | 4505842..4506120 | + | 279 | WP_001296612.1 | hypothetical protein | - |
ECOLIN_RS22430 (ECOLIN_21690) | 4506516..4506734 | + | 219 | WP_001314326.1 | ribbon-helix-helix domain-containing protein | - |
ECOLIN_RS22435 (ECOLIN_21695) | 4506950..4507879 | - | 930 | WP_000027720.1 | formate dehydrogenase accessory protein FdhE | - |
ECOLIN_RS22440 (ECOLIN_21700) | 4507876..4508511 | - | 636 | WP_000829013.1 | formate dehydrogenase cytochrome b556 subunit | - |
ECOLIN_RS22445 (ECOLIN_21705) | 4508508..4509410 | - | 903 | WP_000331377.1 | formate dehydrogenase O subunit beta | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
flank | IS/Tn | - | - | 4503424..4504644 | 1220 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12203.19 Da Isoelectric Point: 9.7791
>T47049 WP_000897302.1 NZ_CP007799:4504881-4505192 [Escherichia coli Nissle 1917]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW
Download Length: 312 bp
>T47049 NZ_CP007799:4504881-4505192 [Escherichia coli Nissle 1917]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
Antitoxin
Download Length: 97 a.a. Molecular weight: 10846.43 Da Isoelectric Point: 10.8907
>AT47049 WP_000356397.1 NZ_CP007799:4505193-4505483 [Escherichia coli Nissle 1917]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR
Download Length: 291 bp
>AT47049 NZ_CP007799:4505193-4505483 [Escherichia coli Nissle 1917]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
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