Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4504881..4505483 Replicon chromosome
Accession NZ_CP007799
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag ECOLIN_RS22410 Protein ID WP_000897302.1
Coordinates 4504881..4505192 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ECOLIN_RS22415 Protein ID WP_000356397.1
Coordinates 4505193..4505483 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECOLIN_RS22385 (ECOLIN_21650) 4500363..4500800 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
ECOLIN_RS22390 (ECOLIN_21655) 4500845..4501786 + 942 WP_024199419.1 fatty acid biosynthesis protein FabY -
ECOLIN_RS22395 (ECOLIN_21660) 4501850..4502758 - 909 WP_001305059.1 alpha/beta hydrolase -
ECOLIN_RS22400 (ECOLIN_21665) 4502887..4503405 + 519 WP_000115885.1 ClbS/DfsB family four-helix bundle protein -
ECOLIN_RS22405 (ECOLIN_21670) 4503424..4504644 + 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
ECOLIN_RS22410 (ECOLIN_21675) 4504881..4505192 + 312 WP_000897302.1 hypothetical protein Toxin
ECOLIN_RS22415 (ECOLIN_21680) 4505193..4505483 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
ECOLIN_RS22425 4505842..4506120 + 279 WP_001296612.1 hypothetical protein -
ECOLIN_RS22430 (ECOLIN_21690) 4506516..4506734 + 219 WP_001314326.1 ribbon-helix-helix domain-containing protein -
ECOLIN_RS22435 (ECOLIN_21695) 4506950..4507879 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
ECOLIN_RS22440 (ECOLIN_21700) 4507876..4508511 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
ECOLIN_RS22445 (ECOLIN_21705) 4508508..4509410 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4503424..4504644 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T47049 WP_000897302.1 NZ_CP007799:4504881-4505192 [Escherichia coli Nissle 1917]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T47049 NZ_CP007799:4504881-4505192 [Escherichia coli Nissle 1917]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT47049 WP_000356397.1 NZ_CP007799:4505193-4505483 [Escherichia coli Nissle 1917]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT47049 NZ_CP007799:4505193-4505483 [Escherichia coli Nissle 1917]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References