Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2324375..2325206 Replicon chromosome
Accession NZ_CP035486
Organism Escherichia coli strain NissleGFP_pZE21

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag EUJ32_RS11590 Protein ID WP_000854814.1
Coordinates 2324832..2325206 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag EUJ32_RS11585 Protein ID WP_001285586.1
Coordinates 2324375..2324743 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EUJ32_RS11545 2319592..2320662 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
EUJ32_RS11550 2320798..2321475 + 678 WP_001362823.1 hypothetical protein -
EUJ32_RS11555 2321491..2321901 + 411 WP_000846704.1 hypothetical protein -
EUJ32_RS11565 2322122..2322943 + 822 WP_001234569.1 DUF945 domain-containing protein -
EUJ32_RS11570 2323034..2323519 + 486 WP_000213703.1 antirestriction protein -
EUJ32_RS11575 2323535..2324011 + 477 WP_001351157.1 RadC family protein -
EUJ32_RS11580 2324080..2324301 + 222 WP_000692323.1 DUF987 domain-containing protein -
EUJ32_RS11585 2324375..2324743 + 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
EUJ32_RS11590 2324832..2325206 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
EUJ32_RS11595 2325203..2325397 + 195 WP_000988601.1 hypothetical protein -
EUJ32_RS11600 2325410..2325523 + 114 WP_001161660.1 DUF957 domain-containing protein -
EUJ32_RS11605 2325812..2325958 - 147 Protein_2198 transposase domain-containing protein -
EUJ32_RS11610 2326012..2326194 + 183 WP_001016348.1 hypothetical protein -
EUJ32_RS11615 2326295..2326624 - 330 WP_000450409.1 DUF496 family protein -
EUJ32_RS11620 2326796..2327854 - 1059 WP_001200890.1 FUSC family protein -
EUJ32_RS11625 2328052..2328525 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
EUJ32_RS11630 2328582..2329802 - 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2328582..2329802 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T117959 WP_000854814.1 NZ_CP035486:2324832-2325206 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T117959 NZ_CP035486:2324832-2325206 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT117959 WP_001285586.1 NZ_CP035486:2324375-2324743 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT117959 NZ_CP035486:2324375-2324743 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References