Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4841061..4841663 Replicon chromosome
Accession NZ_CP080246
Organism Escherichia coli strain SYNB1934

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag KZX95_RS23140 Protein ID WP_000897302.1
Coordinates 4841352..4841663 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KZX95_RS23135 Protein ID WP_000356397.1
Coordinates 4841061..4841351 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX95_RS23110 (4837134) 4837134..4838036 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
KZX95_RS23115 (4838033) 4838033..4838668 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
KZX95_RS23120 (4838665) 4838665..4839594 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
KZX95_RS23125 (4839810) 4839810..4840028 - 219 WP_001314326.1 CopG family transcriptional regulator -
KZX95_RS23130 (4840424) 4840424..4840702 - 279 WP_001296612.1 hypothetical protein -
KZX95_RS23135 (4841061) 4841061..4841351 - 291 WP_000356397.1 NadS family protein Antitoxin
KZX95_RS23140 (4841352) 4841352..4841663 - 312 WP_000897302.1 hypothetical protein Toxin
KZX95_RS23145 (4841900) 4841900..4843120 - 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
KZX95_RS23150 (4843139) 4843139..4843657 - 519 WP_000115885.1 ClbS/DfsB family four-helix bundle protein -
KZX95_RS23155 (4843786) 4843786..4844694 + 909 WP_001305059.1 alpha/beta hydrolase -
KZX95_RS23160 (4844758) 4844758..4845699 - 942 WP_024199419.1 fatty acid biosynthesis protein FabY -
KZX95_RS23165 (4845744) 4845744..4846181 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4841900..4843120 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T211374 WP_000897302.1 NZ_CP080246:c4841663-4841352 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T211374 NZ_CP104848:c4240517-4240110 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT211374 WP_000356397.1 NZ_CP080246:c4841351-4841061 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT211374 NZ_CP104848:c4240764-4240498 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References