Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 2864232..2864834 Replicon chromosome
Accession NZ_CP069630
Organism Escherichia coli strain ESM2.2

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag JSQ94_RS13625 Protein ID WP_000897302.1
Coordinates 2864232..2864543 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JSQ94_RS13630 Protein ID WP_000356397.1
Coordinates 2864544..2864834 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ94_RS13600 (2859714) 2859714..2860151 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
JSQ94_RS13605 (2860196) 2860196..2861137 + 942 WP_024199419.1 fatty acid biosynthesis protein FabY -
JSQ94_RS13610 (2861201) 2861201..2862109 - 909 WP_001305059.1 alpha/beta hydrolase -
JSQ94_RS13615 (2862238) 2862238..2862756 + 519 WP_000115885.1 ClbS/DfsB family four-helix bundle protein -
JSQ94_RS13620 (2862775) 2862775..2863995 + 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
JSQ94_RS13625 (2864232) 2864232..2864543 + 312 WP_000897302.1 hypothetical protein Toxin
JSQ94_RS13630 (2864544) 2864544..2864834 + 291 WP_000356397.1 NadS family protein Antitoxin
JSQ94_RS13635 (2865193) 2865193..2865471 + 279 WP_001296612.1 hypothetical protein -
JSQ94_RS13640 (2865867) 2865867..2866085 + 219 WP_001314326.1 CopG family transcriptional regulator -
JSQ94_RS13645 (2866301) 2866301..2867230 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
JSQ94_RS13650 (2867227) 2867227..2867862 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
JSQ94_RS13655 (2867859) 2867859..2868761 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2862775..2863995 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T191373 WP_000897302.1 NZ_CP069630:2864232-2864543 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T191373 NZ_CP091691:4278198-4278512 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT191373 WP_000356397.1 NZ_CP069630:2864544-2864834 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT191373 NZ_CP091691:4278509-4278925 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References