211354

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1924498..1925329 Replicon chromosome
Accession NZ_CP080246
Organism Escherichia coli strain SYNB1934

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag KZX95_RS09065 Protein ID WP_000854814.1
Coordinates 1924498..1924872 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag KZX95_RS09070 Protein ID WP_001285586.1
Coordinates 1924961..1925329 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KZX95_RS09025 (1919902) 1919902..1921122 + 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
KZX95_RS09030 (1921179) 1921179..1921652 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
KZX95_RS09035 (1921850) 1921850..1922908 + 1059 WP_001200890.1 FUSC family protein -
KZX95_RS09040 (1923080) 1923080..1923409 + 330 WP_000450409.1 DUF496 family protein -
KZX95_RS09045 (1923510) 1923510..1923833 - 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
KZX95_RS09050 (1923812) 1923812..1923892 + 81 WP_023441679.1 hypothetical protein -
KZX95_RS09055 (1924181) 1924181..1924261 - 81 Protein_1773 hypothetical protein -
KZX95_RS09060 (1924307) 1924307..1924501 - 195 WP_000988601.1 DUF5983 family protein -
KZX95_RS09065 (1924498) 1924498..1924872 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
KZX95_RS09070 (1924961) 1924961..1925329 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
KZX95_RS09075 (1925403) 1925403..1925624 - 222 WP_000692323.1 DUF987 domain-containing protein -
KZX95_RS09080 (1925693) 1925693..1926169 - 477 WP_001351157.1 RadC family protein -
KZX95_RS09085 (1926185) 1926185..1926670 - 486 WP_000213703.1 antirestriction protein -
KZX95_RS09090 (1926761) 1926761..1927582 - 822 WP_001234569.1 DUF932 domain-containing protein -
KZX95_RS09095 (1927803) 1927803..1928213 - 411 WP_000846704.1 hypothetical protein -
KZX95_RS09100 (1928229) 1928229..1928906 - 678 WP_001362823.1 hypothetical protein -
KZX95_RS09105 (1929042) 1929042..1930112 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1919902..1921122 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T211354 WP_000854814.1 NZ_CP080246:c1924872-1924498 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T211354 NZ_CP104847:35070-35375 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT211354 WP_001285586.1 NZ_CP080246:c1925329-1924961 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT211354 NZ_CP104847:34850-35068 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References