Detailed information of TA system TA242135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 239190..239792
• Replicon: chromosome
• Accession: NZ_CP095514
• Organism: Escherichia coli strain GN02323

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: MW403_RS01145
• Protein ID: WP_000897302.1
• Coordinates: 239190..239501 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MW403_RS01150
• Protein ID: WP_000356397.1
• Coordinates: 239502..239792 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW403_RS01120 (234672)	234672..235109	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
MW403_RS01125 (235154)	235154..236095	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MW403_RS01130 (236159)	236159..237067	-	909	WP_001305059.1	alpha/beta hydrolase	-
MW403_RS01135 (237125)	237125..238345	-	1221	WP_000343765.1	ISL3-like element ISEc53 family transposase	-
MW403_RS01140 (238364)	238364..238882	-	519	WP_000115885.1	ClbS/DfsB family four-helix bundle protein	-
MW403_RS01145 (239190)	239190..239501	+	312	WP_000897302.1	hypothetical protein	Toxin
MW403_RS01150 (239502)	239502..239792	+	291	WP_000356397.1	NadS family protein	Antitoxin
MW403_RS01155 (240151)	240151..240429	+	279	WP_001296612.1	hypothetical protein	-
MW403_RS01160 (240825)	240825..241043	+	219	WP_001314326.1	CopG family transcriptional regulator	-
MW403_RS01165 (241259)	241259..242188	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
MW403_RS01170 (242185)	242185..242820	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MW403_RS01175 (242817)	242817..243719	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP007799 Escherichia coli Nissle 1917 IS/Tn TA047049 4503424..4504644 0	flank	IS/Tn	-	-	237125..238345	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T242135 WP_000897302.1 NZ_CP095514:239190-239501 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T242135 NZ_OW052188:89068-89361 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT242135 WP_000356397.1 NZ_CP095514:239502-239792 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT242135 NZ_OW052188:88802-89071 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: S1P416