Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1931704..1932535 Replicon chromosome
Accession NZ_AP022533
Organism Escherichia coli strain THO-006

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag JLF32_RS09065 Protein ID WP_000854814.1
Coordinates 1931704..1932078 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag JLF32_RS09070 Protein ID WP_001285586.1
Coordinates 1932167..1932535 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF32_RS09025 1927108..1928328 + 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
JLF32_RS09030 1928385..1928858 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
JLF32_RS09035 1929056..1930114 + 1059 WP_001200890.1 FUSC family protein -
JLF32_RS09040 1930286..1930615 + 330 WP_000450409.1 DUF496 family protein -
JLF32_RS09045 1930716..1930898 - 183 WP_001016348.1 hypothetical protein -
JLF32_RS09050 1930925..1931098 + 174 WP_032082634.1 transposase domain-containing protein -
JLF32_RS09055 1931387..1931500 - 114 WP_001161660.1 DUF957 domain-containing protein -
JLF32_RS09060 1931513..1931707 - 195 WP_000988601.1 hypothetical protein -
JLF32_RS09065 1931704..1932078 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
JLF32_RS09070 1932167..1932535 - 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
JLF32_RS09075 1932609..1932830 - 222 WP_000692323.1 DUF987 domain-containing protein -
JLF32_RS09080 1932899..1933375 - 477 WP_001351157.1 RadC family protein -
JLF32_RS09085 1933391..1933876 - 486 WP_000213703.1 antirestriction protein -
JLF32_RS09090 1933967..1934788 - 822 WP_001234569.1 DUF945 domain-containing protein -
JLF32_RS09095 1935009..1935419 - 411 WP_000846704.1 hypothetical protein -
JLF32_RS09100 1935435..1936112 - 678 WP_001362823.1 hypothetical protein -
JLF32_RS09105 1936248..1937318 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1927108..1928328 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T35969 WP_000854814.1 NZ_AP022533:c1932078-1931704 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T35969 NZ_AP022533:c1932078-1931704 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT35969 WP_001285586.1 NZ_AP022533:c1932535-1932167 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT35969 NZ_AP022533:c1932535-1932167 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References