Detailed information of TA system TA190498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1749304..1749906
• Replicon: chromosome
• Accession: NZ_CP069453
• Organism: Escherichia coli strain FDAARGOS_1254

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: I6J84_RS08225
• Protein ID: WP_000897302.1
• Coordinates: 1749304..1749615 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6J84_RS08230
• Protein ID: WP_000356397.1
• Coordinates: 1749616..1749906 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6J84_RS08200	1744787..1745224	+	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6J84_RS08205	1745269..1746210	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
I6J84_RS08210	1746274..1747182	-	909	WP_001331553.1	alpha/beta hydrolase	-
I6J84_RS08215	1747310..1747828	+	519	WP_000115885.1	ClbS/DfsB family four-helix bundle protein	-
I6J84_RS08220	1747847..1749067	+	1221	WP_000343766.1	ISL3-like element ISEc53 family transposase	-
I6J84_RS08225	1749304..1749615	+	312	WP_000897302.1	hypothetical protein	Toxin
I6J84_RS08230	1749616..1749906	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6J84_RS08235	1750265..1750543	+	279	WP_001296612.1	hypothetical protein	-
I6J84_RS08240	1750939..1751157	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
I6J84_RS08245	1751373..1752302	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
I6J84_RS08250	1752299..1752934	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6J84_RS08255	1752931..1753833	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP007799 Escherichia coli Nissle 1917 IS/Tn TA047049 4503424..4504644 0.001	flank	IS/Tn	-	-	1747847..1749067	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T190498 WP_000897302.1 NZ_CP069453:1749304-1749615 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T190498 NZ_CP091375:2972634-2972816 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTAACAATCCCTCATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT190498 WP_000356397.1 NZ_CP069453:1749616-1749906 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT190498 NZ_CP091375:2972881-2973285 [Acinetobacter baumannii]
ATGTTATATCCAATTGCTATTGAAAGAGGTACAGACACCGAAGCCTTTGGTGTCTCCGTTCCAGATATTCCAGGGTGTTT
CAGCGCAGGCGATACATTATATGAAGCTATCGAGAATGTTAAAGAGGCTATTTCTGGCCACTTGGAAATCCTAGCAGAAG
ATGGAGAGGAGATTCCTTTAGCATCTGATGTCAGTAAGTTTATTGACCAAGAAGATTATAGAGGTATGATCTGGGCAGTT
ACTGAAGTTGATGTTAGCCGTTACTTAGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGATTAATTCGGAAGAT
TGATGATAATGTTGGTAAAGATAAAAGATTTAAAACTCGCTCTGCTTTTTTGGCCGCTGGTGCTGAAAAGCTACTACATG
CTTAA

Structures

Toxin

• AlphaFold DB: S1P416