Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 69059..69890 Replicon chromosome
Accession NZ_CP037449
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag E0708_RS00295 Protein ID WP_000854814.1
Coordinates 69516..69890 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag E0708_RS00290 Protein ID WP_001285586.1
Coordinates 69059..69427 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E0708_RS00255 64276..65346 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
E0708_RS00260 65482..66159 + 678 WP_001362823.1 hypothetical protein -
E0708_RS00265 66175..66585 + 411 WP_000846704.1 hypothetical protein -
E0708_RS00270 66806..67627 + 822 WP_001234569.1 DUF945 domain-containing protein -
E0708_RS00275 67718..68203 + 486 WP_000213703.1 antirestriction protein -
E0708_RS00280 68219..68695 + 477 WP_001351157.1 RadC family protein -
E0708_RS00285 68764..68985 + 222 WP_000692323.1 DUF987 domain-containing protein -
E0708_RS00290 69059..69427 + 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
E0708_RS00295 69516..69890 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
E0708_RS00300 69887..70081 + 195 WP_000988601.1 hypothetical protein -
E0708_RS00305 70094..70207 + 114 WP_001161660.1 DUF957 domain-containing protein -
E0708_RS00310 70496..70669 - 174 WP_032082634.1 transposase domain-containing protein -
E0708_RS00315 70696..70878 + 183 WP_001016348.1 hypothetical protein -
E0708_RS00320 70979..71308 - 330 WP_000450409.1 DUF496 family protein -
E0708_RS00325 71480..72538 - 1059 WP_001200890.1 FUSC family protein -
E0708_RS00330 72736..73209 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
E0708_RS00335 73266..74486 - 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 73266..74486 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T119953 WP_000854814.1 NZ_CP037449:69516-69890 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T119953 NZ_CP037449:69516-69890 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT119953 WP_001285586.1 NZ_CP037449:69059-69427 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT119953 NZ_CP037449:69059-69427 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References