Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 726692..727523 Replicon chromosome
Accession NZ_CP022686
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag CIW80_RS03775 Protein ID WP_000854814.1
Coordinates 727149..727523 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag CIW80_RS03770 Protein ID WP_001285586.1
Coordinates 726692..727060 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CIW80_RS03730 721909..722979 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
CIW80_RS03735 723115..723792 + 678 WP_001362823.1 hypothetical protein -
CIW80_RS03740 723808..724218 + 411 WP_000846704.1 hypothetical protein -
CIW80_RS03750 724439..725260 + 822 WP_001234569.1 DUF945 domain-containing protein -
CIW80_RS03755 725351..725836 + 486 WP_000213703.1 antirestriction protein -
CIW80_RS03760 725852..726328 + 477 WP_001351157.1 RadC family protein -
CIW80_RS03765 726397..726618 + 222 WP_000692323.1 DUF987 domain-containing protein -
CIW80_RS03770 726692..727060 + 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
CIW80_RS03775 727149..727523 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
CIW80_RS03780 727520..727714 + 195 WP_000988601.1 hypothetical protein -
CIW80_RS03785 727727..727840 + 114 WP_001161660.1 DUF957 domain-containing protein -
CIW80_RS03790 728129..728275 - 147 Protein_690 transposase domain-containing protein -
CIW80_RS03795 728329..728511 + 183 WP_001016348.1 hypothetical protein -
CIW80_RS03800 728612..728941 - 330 WP_000450409.1 DUF496 family protein -
CIW80_RS03805 729113..730171 - 1059 WP_001200890.1 FUSC family protein -
CIW80_RS03810 730369..730842 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
CIW80_RS03815 730899..732119 - 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 730899..732119 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T79533 WP_000854814.1 NZ_CP022686:727149-727523 [Escherichia coli Nissle 1917]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T79533 NZ_CP022686:727149-727523 [Escherichia coli Nissle 1917]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT79533 WP_001285586.1 NZ_CP022686:726692-727060 [Escherichia coli Nissle 1917]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT79533 NZ_CP022686:726692-727060 [Escherichia coli Nissle 1917]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References