Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1917429..1918260 Replicon chromosome
Accession NZ_CP058217
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HW372_RS09040 Protein ID WP_000854814.1
Coordinates 1917429..1917803 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag HW372_RS09045 Protein ID WP_001285586.1
Coordinates 1917892..1918260 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW372_RS09000 (1912833) 1912833..1914053 + 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
HW372_RS09005 (1914110) 1914110..1914583 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
HW372_RS09010 (1914781) 1914781..1915839 + 1059 WP_001200890.1 FUSC family protein -
HW372_RS09015 (1916011) 1916011..1916340 + 330 WP_000450409.1 DUF496 family protein -
HW372_RS09020 (1916441) 1916441..1916764 - 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
HW372_RS09025 (1916743) 1916743..1916823 + 81 WP_023441679.1 hypothetical protein -
HW372_RS09030 (1917112) 1917112..1917192 - 81 Protein_1769 hypothetical protein -
HW372_RS09035 (1917238) 1917238..1917432 - 195 WP_000988601.1 DUF5983 family protein -
HW372_RS09040 (1917429) 1917429..1917803 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
HW372_RS09045 (1917892) 1917892..1918260 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
HW372_RS09050 (1918334) 1918334..1918555 - 222 WP_000692323.1 DUF987 domain-containing protein -
HW372_RS09055 (1918624) 1918624..1919100 - 477 WP_001351157.1 RadC family protein -
HW372_RS09060 (1919116) 1919116..1919601 - 486 WP_000213703.1 antirestriction protein -
HW372_RS09065 (1919692) 1919692..1920513 - 822 WP_001234569.1 DUF932 domain-containing protein -
HW372_RS09070 (1920734) 1920734..1921144 - 411 WP_000846704.1 hypothetical protein -
HW372_RS09075 (1921160) 1921160..1921837 - 678 WP_001362823.1 hypothetical protein -
HW372_RS09080 (1921973) 1921973..1923043 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1912833..1914053 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T166179 WP_000854814.1 NZ_CP058217:c1917803-1917429 [Escherichia coli Nissle 1917]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T166179 NZ_CP074052:c3999791-3999393 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT166179 WP_001285586.1 NZ_CP058217:c1918260-1917892 [Escherichia coli Nissle 1917]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT166179 NZ_CP074052:c4000087-3999794 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References