Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 1917429..1918260 | Replicon | chromosome |
| Accession | NZ_CP058217 | ||
| Organism | Escherichia coli Nissle 1917 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | S1P968 |
| Locus tag | HW372_RS09040 | Protein ID | WP_000854814.1 |
| Coordinates | 1917429..1917803 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | S1PWQ3 |
| Locus tag | HW372_RS09045 | Protein ID | WP_001285586.1 |
| Coordinates | 1917892..1918260 (-) | Length | 123 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HW372_RS09000 (1912833) | 1912833..1914053 | + | 1221 | WP_000343765.1 | ISL3-like element ISEc53 family transposase | - |
| HW372_RS09005 (1914110) | 1914110..1914583 | + | 474 | WP_001105376.1 | DNA gyrase inhibitor SbmC | - |
| HW372_RS09010 (1914781) | 1914781..1915839 | + | 1059 | WP_001200890.1 | FUSC family protein | - |
| HW372_RS09015 (1916011) | 1916011..1916340 | + | 330 | WP_000450409.1 | DUF496 family protein | - |
| HW372_RS09020 (1916441) | 1916441..1916764 | - | 324 | WP_223216378.1 | EutP/PduV family microcompartment system protein | - |
| HW372_RS09025 (1916743) | 1916743..1916823 | + | 81 | WP_023441679.1 | hypothetical protein | - |
| HW372_RS09030 (1917112) | 1917112..1917192 | - | 81 | Protein_1769 | hypothetical protein | - |
| HW372_RS09035 (1917238) | 1917238..1917432 | - | 195 | WP_000988601.1 | DUF5983 family protein | - |
| HW372_RS09040 (1917429) | 1917429..1917803 | - | 375 | WP_000854814.1 | type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA | Toxin |
| HW372_RS09045 (1917892) | 1917892..1918260 | - | 369 | WP_001285586.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| HW372_RS09050 (1918334) | 1918334..1918555 | - | 222 | WP_000692323.1 | DUF987 domain-containing protein | - |
| HW372_RS09055 (1918624) | 1918624..1919100 | - | 477 | WP_001351157.1 | RadC family protein | - |
| HW372_RS09060 (1919116) | 1919116..1919601 | - | 486 | WP_000213703.1 | antirestriction protein | - |
| HW372_RS09065 (1919692) | 1919692..1920513 | - | 822 | WP_001234569.1 | DUF932 domain-containing protein | - |
| HW372_RS09070 (1920734) | 1920734..1921144 | - | 411 | WP_000846704.1 | hypothetical protein | - |
| HW372_RS09075 (1921160) | 1921160..1921837 | - | 678 | WP_001362823.1 | hypothetical protein | - |
| HW372_RS09080 (1921973) | 1921973..1923043 | - | 1071 | WP_000102631.1 | patatin-like phospholipase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| flank | IS/Tn | - | - | 1912833..1914053 | 1220 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 13898.94 Da Isoelectric Point: 7.8522
>T166179 WP_000854814.1 NZ_CP058217:c1917803-1917429 [Escherichia coli Nissle 1917]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T166179 NZ_CP074052:c3999791-3999393 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 123 a.a. Molecular weight: 13563.41 Da Isoelectric Point: 5.0468
>AT166179 WP_001285586.1 NZ_CP058217:c1918260-1917892 [Escherichia coli Nissle 1917]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN
Download Length: 369 bp
>AT166179 NZ_CP074052:c4000087-3999794 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829FY50 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1PWQ3 |