Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 726643..727474 Replicon chromosome
Accession NZ_CP069636
Organism Escherichia coli strain SSGw2

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag JSQ96_RS03530 Protein ID WP_000854814.1
Coordinates 727100..727474 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag JSQ96_RS03525 Protein ID WP_001285586.1
Coordinates 726643..727011 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ96_RS03490 (721860) 721860..722930 + 1071 WP_000102631.1 patatin-like phospholipase family protein -
JSQ96_RS03495 (723066) 723066..723743 + 678 WP_001362823.1 hypothetical protein -
JSQ96_RS03500 (723759) 723759..724169 + 411 WP_000846704.1 hypothetical protein -
JSQ96_RS03505 (724390) 724390..725211 + 822 WP_001234569.1 DUF932 domain-containing protein -
JSQ96_RS03510 (725302) 725302..725787 + 486 WP_000213703.1 antirestriction protein -
JSQ96_RS03515 (725803) 725803..726279 + 477 WP_001351157.1 RadC family protein -
JSQ96_RS03520 (726348) 726348..726569 + 222 WP_000692323.1 DUF987 domain-containing protein -
JSQ96_RS03525 (726643) 726643..727011 + 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
JSQ96_RS03530 (727100) 727100..727474 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
JSQ96_RS03535 (727471) 727471..727665 + 195 WP_000988601.1 DUF5983 family protein -
JSQ96_RS03540 (727711) 727711..727791 + 81 Protein_696 hypothetical protein -
JSQ96_RS03545 (728080) 728080..728160 - 81 WP_023441679.1 hypothetical protein -
JSQ96_RS03550 (728139) 728139..728462 + 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
JSQ96_RS03555 (728563) 728563..728892 - 330 WP_000450409.1 DUF496 family protein -
JSQ96_RS03560 (729064) 729064..730122 - 1059 WP_001200890.1 FUSC family protein -
JSQ96_RS03565 (730320) 730320..730793 - 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
JSQ96_RS03570 (730850) 730850..732070 - 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 730850..732070 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T191421 WP_000854814.1 NZ_CP069636:727100-727474 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T191421 NZ_CP091703:3269489-3269800 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT191421 WP_001285586.1 NZ_CP069636:726643-727011 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT191421 NZ_CP091703:3269801-3270091 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References