Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2814112..2814943 Replicon chromosome
Accession NZ_CP055251
Organism Escherichia coli strain AH01

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HUU83_RS13700 Protein ID WP_000854814.1
Coordinates 2814569..2814943 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A376RIF3
Locus tag HUU83_RS13695 Protein ID WP_053882193.1
Coordinates 2814112..2814480 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUU83_RS13660 2810468..2810815 - 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
HUU83_RS13665 2810815..2811492 - 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -
HUU83_RS13670 2811550..2811783 + 234 WP_001119729.1 DUF905 family protein -
HUU83_RS13675 2811883..2812701 + 819 WP_001234629.1 DUF945 domain-containing protein -
HUU83_RS13680 2812783..2813262 + 480 WP_000860087.1 antirestriction protein -
HUU83_RS13685 2813278..2813754 + 477 WP_001186726.1 RadC family protein -
HUU83_RS13690 2813817..2814038 + 222 WP_000692323.1 DUF987 domain-containing protein -
HUU83_RS13695 2814112..2814480 + 369 WP_053882193.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
HUU83_RS13700 2814569..2814943 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
HUU83_RS13705 2814940..2815134 + 195 WP_039023483.1 hypothetical protein -
HUU83_RS13710 2815147..2815260 + 114 WP_001161660.1 DUF957 domain-containing protein -
HUU83_RS13715 2815549..2815677 - 129 Protein_2679 transposase domain-containing protein -
HUU83_RS13720 2815749..2815931 + 183 WP_001016348.1 hypothetical protein -
HUU83_RS13725 2816032..2816361 - 330 WP_000450409.1 DUF496 family protein -
HUU83_RS13730 2816533..2817591 - 1059 WP_001200891.1 FUSC family protein -
HUU83_RS13735 2817789..2818262 - 474 WP_176285482.1 DNA gyrase inhibitor SbmC -
HUU83_RS13740 2818390..2818908 + 519 WP_000115885.1 ClbS/DfsB family four-helix bundle protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2818927..2820147 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T162106 WP_000854814.1 NZ_CP055251:2814569-2814943 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T162106 NZ_CP071523:25229-25534 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13597.47 Da        Isoelectric Point: 6.3139

>AT162106 WP_053882193.1 NZ_CP055251:2814112-2814480 [Escherichia coli]
VSDTLPGTTLPDDNHDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT162106 NZ_CP071523:25009-25227 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A376RIF3

References