Detailed information of TA system TA079540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2864275..2864877
• Replicon: chromosome
• Accession: NZ_CP022686
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: CIW80_RS14330
• Protein ID: WP_000897302.1
• Coordinates: 2864275..2864586 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CIW80_RS14335
• Protein ID: WP_000356397.1
• Coordinates: 2864587..2864877 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIW80_RS14305	2859757..2860194	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CIW80_RS14310	2860239..2861180	+	942	WP_024199419.1	fatty acid biosynthesis protein FabY	-
CIW80_RS14315	2861244..2862152	-	909	WP_001305059.1	alpha/beta hydrolase	-
CIW80_RS14320	2862281..2862799	+	519	WP_000115885.1	ClbS/DfsB family four-helix bundle protein	-
CIW80_RS14325	2862818..2864038	+	1221	WP_000343765.1	ISL3-like element ISEc53 family transposase	-
CIW80_RS14330	2864275..2864586	+	312	WP_000897302.1	hypothetical protein	Toxin
CIW80_RS14335	2864587..2864877	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CIW80_RS14345	2865236..2865514	+	279	WP_001296612.1	hypothetical protein	-
CIW80_RS14355	2865910..2866128	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
CIW80_RS14360	2866344..2867273	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CIW80_RS14365	2867270..2867905	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CIW80_RS14370	2867902..2868804	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP007799 Escherichia coli Nissle 1917 IS/Tn TA047049 4503424..4504644 0	flank	IS/Tn	-	-	2862818..2864038	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T79540 WP_000897302.1 NZ_CP022686:2864275-2864586 [Escherichia coli Nissle 1917]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T79540 NZ_CP022686:2864275-2864586 [Escherichia coli Nissle 1917]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT79540 WP_000356397.1 NZ_CP022686:2864587-2864877 [Escherichia coli Nissle 1917]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT79540 NZ_CP022686:2864587-2864877 [Escherichia coli Nissle 1917]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416