166199

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4835477..4836079 Replicon chromosome
Accession NZ_CP058217
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag HW372_RS23125 Protein ID WP_000897302.1
Coordinates 4835768..4836079 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HW372_RS23120 Protein ID WP_000356397.1
Coordinates 4835477..4835767 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW372_RS23095 (4831550) 4831550..4832452 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
HW372_RS23100 (4832449) 4832449..4833084 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HW372_RS23105 (4833081) 4833081..4834010 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
HW372_RS23110 (4834226) 4834226..4834444 - 219 WP_001314326.1 CopG family transcriptional regulator -
HW372_RS23115 (4834840) 4834840..4835118 - 279 WP_001296612.1 hypothetical protein -
HW372_RS23120 (4835477) 4835477..4835767 - 291 WP_000356397.1 NadS family protein Antitoxin
HW372_RS23125 (4835768) 4835768..4836079 - 312 WP_000897302.1 hypothetical protein Toxin
HW372_RS23130 (4836316) 4836316..4837536 - 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
HW372_RS23135 (4837555) 4837555..4838073 - 519 WP_000115885.1 ClbS/DfsB family four-helix bundle protein -
HW372_RS23140 (4838202) 4838202..4839110 + 909 WP_001305059.1 alpha/beta hydrolase -
HW372_RS23145 (4839174) 4839174..4840115 - 942 WP_024199419.1 fatty acid biosynthesis protein FabY -
HW372_RS23150 (4840160) 4840160..4840597 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 4836316..4837536 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T166199 WP_000897302.1 NZ_CP058217:c4836079-4835768 [Escherichia coli Nissle 1917]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T166199 NZ_CP074087:1977679-1977781 [Klebsiella pneumoniae subsp. pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT166199 WP_000356397.1 NZ_CP058217:c4835767-4835477 [Escherichia coli Nissle 1917]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT166199 NZ_CP074087:c1977788-1977643 [Klebsiella pneumoniae subsp. pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References