Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1931051..1931882 Replicon chromosome
Accession NZ_CP081010
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag K3072_RS09090 Protein ID WP_000854814.1
Coordinates 1931051..1931425 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag K3072_RS09095 Protein ID WP_001285586.1
Coordinates 1931514..1931882 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS09050 (1926455) 1926455..1927675 + 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
K3072_RS09055 (1927732) 1927732..1928205 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
K3072_RS09060 (1928403) 1928403..1929461 + 1059 WP_001200890.1 FUSC family protein -
K3072_RS09065 (1929633) 1929633..1929962 + 330 WP_000450409.1 DUF496 family protein -
K3072_RS25220 (1930063) 1930063..1930419 - 357 WP_000929389.1 EutP/PduV family microcompartment system protein -
K3072_RS09080 (1930734) 1930734..1930814 - 81 Protein_1787 hypothetical protein -
K3072_RS09085 (1930860) 1930860..1931054 - 195 WP_000988601.1 DUF5983 family protein -
K3072_RS09090 (1931051) 1931051..1931425 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K3072_RS09095 (1931514) 1931514..1931882 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
K3072_RS09100 (1931956) 1931956..1932177 - 222 WP_000692323.1 DUF987 domain-containing protein -
K3072_RS09105 (1932246) 1932246..1932722 - 477 WP_001351157.1 RadC family protein -
K3072_RS09110 (1932738) 1932738..1933223 - 486 WP_000213703.1 antirestriction protein -
K3072_RS09115 (1933314) 1933314..1934135 - 822 WP_001234569.1 DUF932 domain-containing protein -
K3072_RS09120 (1934356) 1934356..1934766 - 411 WP_000846704.1 hypothetical protein -
K3072_RS09125 (1934782) 1934782..1935459 - 678 WP_001362823.1 hypothetical protein -
K3072_RS09130 (1935595) 1935595..1936665 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1926455..1927675 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T212558 WP_000854814.1 NZ_CP081010:c1931425-1931051 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T212558 NZ_CP107274:c963148-962729 [Neisseria gonorrhoeae]
ATGATTTTGCTGGACACGAATGTGATTTCCGAACCTTTGCGCCCACAACCCAATGAACGTGTGGTGGCATGGTTGGATAG
TTTGATATTGGAAGATGTGTATTTGTCTGCCATTACTGTTGCAGAATTGCGTTTGGGTGTGGCGTTGTTGCTCAATGGCA
AGAAAAAGAATGTGCTGCACGAACGTTTGGAACAATCCATTTTGCCTTTATTTGCGGGGCGGATTCTGCCTTTTGATGAA
CCGGTTGCCGCAATCTATGCGCAAATTCGTTCCTATGCCAAAACACATGGCAAAGAGATTGCTGCCGCAGACGGCTATAT
TGCCGCCACTGCAAAACAGCACAGTTTGACAGTTGCTACGCGTGATACCGGCTCATTTTTTGCGGCCGATGTCGCGGTGT
TCAATCCGTGGCACGATTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT212558 WP_001285586.1 NZ_CP081010:c1931882-1931514 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT212558 NZ_CP107274:c963384-963148 [Neisseria gonorrhoeae]
ATGGCTTCTGTTGTGATTAGAAATTTATCCGAGGCCACGCACAACGCAATCAAATTCCGTGCGCGAGCCGCAGGGCGCAG
TACCGAAGCAGAAATCCGCTTAATTTTGGATAACATCGCCAAAGCACAACAAACTGTACGTTTGGGGTCAATGTTGGCAT
CAATAGGGCAGGAAATCGGAGGTGTTGAGCTGGAAGACGTACGCGGTCGTAATACTGATAACGAGGTTTCTTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References