Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2326283..2327114 Replicon chromosome
Accession NZ_CP048304
Organism Escherichia coli strain 9

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag GZS17_RS10855 Protein ID WP_000854814.1
Coordinates 2326283..2326657 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag GZS17_RS10860 Protein ID WP_001285586.1
Coordinates 2326746..2327114 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZS17_RS10815 2322318..2322836 - 519 WP_000115885.1 ClbS/DfsB family four-helix bundle protein -
GZS17_RS10820 2322964..2323437 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
GZS17_RS10825 2323635..2324693 + 1059 WP_001200890.1 FUSC family protein -
GZS17_RS10830 2324865..2325194 + 330 WP_000450409.1 DUF496 family protein -
GZS17_RS10835 2325295..2325477 - 183 WP_001016348.1 hypothetical protein -
GZS17_RS10840 2325531..2325677 + 147 Protein_2102 transposase domain-containing protein -
GZS17_RS10845 2325966..2326079 - 114 WP_001161660.1 DUF957 domain-containing protein -
GZS17_RS10850 2326092..2326286 - 195 WP_000988601.1 hypothetical protein -
GZS17_RS10855 2326283..2326657 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
GZS17_RS10860 2326746..2327114 - 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
GZS17_RS10865 2327188..2327409 - 222 WP_000692323.1 DUF987 domain-containing protein -
GZS17_RS10870 2327478..2327954 - 477 WP_001351157.1 RadC family protein -
GZS17_RS10875 2327970..2328455 - 486 WP_000213703.1 antirestriction protein -
GZS17_RS10880 2328546..2329367 - 822 WP_001234569.1 DUF945 domain-containing protein -
GZS17_RS10885 2329588..2329998 - 411 WP_000846704.1 hypothetical protein -
GZS17_RS10890 2330014..2330691 - 678 WP_001362823.1 hypothetical protein -
GZS17_RS10895 2330827..2331897 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2321079..2322299 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T148966 WP_000854814.1 NZ_CP048304:c2326657-2326283 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T148966 NZ_CP063910:c4643169-4642951 [Klebsiella variicola]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAACTGGCGGTATTTTACTCAGCCGCCGATCATCGTCTGGCTGAAC
TGACCATGAATAAGCTGTACGATAAGATCCCGACTTCAGTCTGGAAGTTTATCCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT148966 WP_001285586.1 NZ_CP048304:c2327114-2326746 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT148966 NZ_CP063910:c4643570-4643196 [Klebsiella variicola]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACTTCGGCTGTCAATCTTCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCGCTTAATTATAAAATTAAGTACACTGAGGATAATAAACTGGTCGCTCAGGTTGACGAATATCTG
GACGATACTTTTACCTTGTTCAGTAACTACGGCATTAACTCAACGGATTTGCAAAAGTGGAAAAAATCCGGTAATCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTCTCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References