Detailed information of TA system TA117966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4461964..4462566
• Replicon: chromosome
• Accession: NZ_CP035486
• Organism: Escherichia coli strain NissleGFP_pZE21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: EUJ32_RS21830
• Protein ID: WP_000897302.1
• Coordinates: 4461964..4462275 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EUJ32_RS21835
• Protein ID: WP_000356397.1
• Coordinates: 4462276..4462566 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EUJ32_RS21805	4457446..4457883	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EUJ32_RS21810	4457928..4458869	+	942	WP_024199419.1	fatty acid biosynthesis protein FabY	-
EUJ32_RS21815	4458933..4459841	-	909	WP_001305059.1	alpha/beta hydrolase	-
EUJ32_RS21820	4459970..4460488	+	519	WP_000115885.1	ClbS/DfsB family four-helix bundle protein	-
EUJ32_RS21825	4460507..4461727	+	1221	WP_000343765.1	ISL3-like element ISEc53 family transposase	-
EUJ32_RS21830	4461964..4462275	+	312	WP_000897302.1	hypothetical protein	Toxin
EUJ32_RS21835	4462276..4462566	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EUJ32_RS21845	4462925..4463203	+	279	WP_001296612.1	hypothetical protein	-
EUJ32_RS21855	4463599..4463817	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
EUJ32_RS21860	4464033..4464962	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
EUJ32_RS21865	4464959..4465594	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EUJ32_RS21870	4465591..4466493	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP007799 Escherichia coli Nissle 1917 IS/Tn TA047049 4503424..4504644 0	flank	IS/Tn	-	-	4460507..4461727	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T117966 WP_000897302.1 NZ_CP035486:4461964-4462275 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T117966 NZ_CP035486:4461964-4462275 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT117966 WP_000356397.1 NZ_CP035486:4462276-4462566 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT117966 NZ_CP035486:4462276-4462566 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416