Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 93549..94380 Replicon chromosome
Accession NZ_CP070041
Organism Escherichia coli strain FDAARGOS_1293

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag I6K23_RS00425 Protein ID WP_000854814.1
Coordinates 93549..93923 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag I6K23_RS00430 Protein ID WP_001285586.1
Coordinates 94012..94380 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6K23_RS00385 (88953) 88953..90173 + 1221 WP_000343766.1 ISL3-like element ISEc53 family transposase -
I6K23_RS00390 (90230) 90230..90703 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
I6K23_RS00395 (90901) 90901..91959 + 1059 WP_001200890.1 FUSC family protein -
I6K23_RS00400 (92131) 92131..92460 + 330 WP_000450409.1 DUF496 family protein -
I6K23_RS24750 (92561) 92561..92917 - 357 WP_000929389.1 EutP/PduV family microcompartment system protein -
I6K23_RS00415 (93232) 93232..93312 - 81 Protein_81 hypothetical protein -
I6K23_RS00420 (93358) 93358..93552 - 195 WP_000988601.1 DUF5983 family protein -
I6K23_RS00425 (93549) 93549..93923 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
I6K23_RS00430 (94012) 94012..94380 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
I6K23_RS00435 (94454) 94454..94675 - 222 WP_000692323.1 DUF987 domain-containing protein -
I6K23_RS00440 (94738) 94738..95214 - 477 WP_204628203.1 RadC family protein -
I6K23_RS00445 (95230) 95230..95715 - 486 WP_000213703.1 antirestriction protein -
I6K23_RS00450 (95806) 95806..96627 - 822 WP_001234569.1 DUF932 domain-containing protein -
I6K23_RS00455 (96848) 96848..97258 - 411 WP_000846704.1 hypothetical protein -
I6K23_RS00460 (97274) 97274..97951 - 678 WP_001362823.1 hypothetical protein -
I6K23_RS00465 (98087) 98087..99157 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 88953..90173 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T192742 WP_000854814.1 NZ_CP070041:c93923-93549 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T192742 NZ_CP092534:c3719982-3719584 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT192742 WP_001285586.1 NZ_CP070041:c94380-94012 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT192742 NZ_CP092534:c3720278-3719985 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References