Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 2864231..2864833 Replicon chromosome
Accession NZ_CP069627
Organism Escherichia coli strain ESM1.4

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag JSQ89_RS13630 Protein ID WP_000897302.1
Coordinates 2864231..2864542 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag JSQ89_RS13635 Protein ID WP_000356397.1
Coordinates 2864543..2864833 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JSQ89_RS13605 (2859713) 2859713..2860150 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
JSQ89_RS13610 (2860195) 2860195..2861136 + 942 WP_024199419.1 fatty acid biosynthesis protein FabY -
JSQ89_RS13615 (2861200) 2861200..2862108 - 909 WP_001305059.1 alpha/beta hydrolase -
JSQ89_RS13620 (2862237) 2862237..2862755 + 519 WP_000115885.1 ClbS/DfsB family four-helix bundle protein -
JSQ89_RS13625 (2862774) 2862774..2863994 + 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
JSQ89_RS13630 (2864231) 2864231..2864542 + 312 WP_000897302.1 hypothetical protein Toxin
JSQ89_RS13635 (2864543) 2864543..2864833 + 291 WP_000356397.1 NadS family protein Antitoxin
JSQ89_RS13640 (2865192) 2865192..2865470 + 279 WP_001296612.1 hypothetical protein -
JSQ89_RS13645 (2865866) 2865866..2866084 + 219 WP_001314326.1 CopG family transcriptional regulator -
JSQ89_RS13650 (2866300) 2866300..2867229 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
JSQ89_RS13655 (2867226) 2867226..2867861 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
JSQ89_RS13660 (2867858) 2867858..2868760 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2862774..2863994 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T191346 WP_000897302.1 NZ_CP069627:2864231-2864542 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T191346 NZ_CP091682:c2838857-2838606 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT191346 WP_000356397.1 NZ_CP069627:2864543-2864833 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT191346 NZ_CP091682:c2838609-2838355 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References