Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3580002..3580833 Replicon chromosome
Accession NZ_CP084096
Organism Escherichia coli O78 strain 3

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag LCF29_RS17285 Protein ID WP_000854814.1
Coordinates 3580459..3580833 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A376RIF3
Locus tag LCF29_RS17280 Protein ID WP_053882193.1
Coordinates 3580002..3580370 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LCF29_RS17245 (3576358) 3576358..3576705 - 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
LCF29_RS17250 (3576705) 3576705..3577382 - 678 WP_001339397.1 IS66-like element accessory protein TnpA -
LCF29_RS17255 (3577440) 3577440..3577673 + 234 WP_001119729.1 DUF905 family protein -
LCF29_RS17260 (3577773) 3577773..3578591 + 819 WP_001234629.1 DUF932 domain-containing protein -
LCF29_RS17265 (3578673) 3578673..3579152 + 480 WP_000860087.1 antirestriction protein -
LCF29_RS17270 (3579168) 3579168..3579644 + 477 WP_001186726.1 RadC family protein -
LCF29_RS17275 (3579707) 3579707..3579928 + 222 WP_000692323.1 DUF987 domain-containing protein -
LCF29_RS17280 (3580002) 3580002..3580370 + 369 WP_053882193.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
LCF29_RS17285 (3580459) 3580459..3580833 + 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
LCF29_RS17290 (3580830) 3580830..3581024 + 195 WP_039023483.1 DUF5983 family protein -
LCF29_RS17295 (3581070) 3581070..3581150 + 81 Protein_3390 hypothetical protein -
LCF29_RS17300 (3581439) 3581439..3581519 - 81 WP_023441679.1 hypothetical protein -
LCF29_RS17305 (3581498) 3581498..3581821 + 324 WP_225343796.1 EutP/PduV family microcompartment system protein -
LCF29_RS17310 (3581922) 3581922..3582251 - 330 WP_000450409.1 DUF496 family protein -
LCF29_RS17315 (3582423) 3582423..3583481 - 1059 WP_001200891.1 FUSC family protein -
LCF29_RS17320 (3583679) 3583679..3584152 - 474 WP_024222062.1 DNA gyrase inhibitor SbmC -
LCF29_RS17325 (3584280) 3584280..3584798 + 519 WP_000115885.1 ClbS/DfsB family four-helix bundle protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3584817..3586037 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T218078 WP_000854814.1 NZ_CP084096:3580459-3580833 [Escherichia coli O78]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T218078 NZ_CP115447:c3803809-3803417 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13597.47 Da        Isoelectric Point: 6.3139

>AT218078 WP_053882193.1 NZ_CP084096:3580002-3580370 [Escherichia coli O78]
VSDTLPGTTLPDDNHDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT218078 NZ_CP115447:c3804084-3803809 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A376RIF3

References