Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2406893..2407724 Replicon chromosome
Accession NZ_CP031256
Organism Escherichia coli strain M16807

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag DV447_RS11985 Protein ID WP_000854814.1
Coordinates 2406893..2407267 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag DV447_RS11990 Protein ID WP_001285586.1
Coordinates 2407356..2407724 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DV447_RS11945 2402297..2403517 + 1221 WP_000343765.1 ISL3-like element ISEc53 family transposase -
DV447_RS11950 2403574..2404047 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
DV447_RS11955 2404245..2405303 + 1059 WP_001200890.1 FUSC family protein -
DV447_RS11960 2405475..2405804 + 330 WP_000450409.1 DUF496 family protein -
DV447_RS11965 2405905..2406087 - 183 WP_001016348.1 hypothetical protein -
DV447_RS11970 2406114..2406287 + 174 WP_032082634.1 transposase domain-containing protein -
DV447_RS11975 2406576..2406689 - 114 WP_001161660.1 DUF957 domain-containing protein -
DV447_RS11980 2406702..2406896 - 195 WP_000988601.1 hypothetical protein -
DV447_RS11985 2406893..2407267 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
DV447_RS11990 2407356..2407724 - 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
DV447_RS11995 2407798..2408019 - 222 WP_000692323.1 DUF987 domain-containing protein -
DV447_RS12000 2408088..2408564 - 477 WP_001351157.1 RadC family protein -
DV447_RS12005 2408580..2409065 - 486 WP_000213703.1 antirestriction protein -
DV447_RS12010 2409156..2409977 - 822 WP_001234569.1 DUF945 domain-containing protein -
DV447_RS12020 2410198..2410608 - 411 WP_000846704.1 hypothetical protein -
DV447_RS12025 2410624..2411301 - 678 WP_001362823.1 hypothetical protein -
DV447_RS12030 2411437..2412507 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 2402297..2403517 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T108747 WP_000854814.1 NZ_CP031256:c2407267-2406893 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T108747 NZ_CP031256:c2407267-2406893 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT108747 WP_001285586.1 NZ_CP031256:c2407724-2407356 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT108747 NZ_CP031256:c2407724-2407356 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References