Detailed information of TA system TA035989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4833945..4834547
• Replicon: chromosome
• Accession: NZ_AP022533
• Organism: Escherichia coli strain THO-006

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: JLF32_RS23150
• Protein ID: WP_000897302.1
• Coordinates: 4834236..4834547 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLF32_RS23145
• Protein ID: WP_000356397.1
• Coordinates: 4833945..4834235 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF32_RS23120	4830018..4830920	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JLF32_RS23125	4830917..4831552	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JLF32_RS23130	4831549..4832478	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
JLF32_RS23135	4832694..4832912	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
JLF32_RS23140	4833308..4833586	-	279	WP_001296612.1	hypothetical protein	-
JLF32_RS23145	4833945..4834235	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JLF32_RS23150	4834236..4834547	-	312	WP_000897302.1	hypothetical protein	Toxin
JLF32_RS23155	4834855..4835373	+	519	WP_000115885.1	ClbS/DfsB family four-helix bundle protein	-
JLF32_RS23160	4835392..4836612	+	1221	WP_000343766.1	ISL3-like element ISEc53 family transposase	-
JLF32_RS23165	4836670..4837578	+	909	WP_001305059.1	alpha/beta hydrolase	-
JLF32_RS23170	4837642..4838583	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JLF32_RS23175	4838628..4839065	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP007799 Escherichia coli Nissle 1917 IS/Tn TA047049 4503424..4504644 0.001	flank	IS/Tn	-	-	4835392..4836612	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T35989 WP_000897302.1 NZ_AP022533:c4834547-4834236 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T35989 NZ_AP022533:c4834547-4834236 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT35989 WP_000356397.1 NZ_AP022533:c4834235-4833945 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT35989 NZ_AP022533:c4834235-4833945 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416