131

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/PHD-PIN
Location 3799243..3799943 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name vapC Uniprot ID O50411
Locus tag Rv3384c Protein ID NP_217901.1
Coordinates 3799243..3799635 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WF13
Locus tag Rv3385c Protein ID NP_217902.1
Coordinates 3799635..3799943 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv3382c 3796448..3797437 - 990 YP_177967.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
Rv3383c 3797437..3798489 - 1053 NP_217900.1 polyprenyl synthetase IdsB -
Rv3384c 3799243..3799635 - 393 NP_217901.1 ribonuclease VapC46 Toxin
Rv3385c 3799635..3799943 - 309 NP_217902.1 antitoxin VapB46 Antitoxin
Rv3386 3800092..3800796 + 705 NP_217903.1 transposase -
Rv3387 3800786..3801463 + 678 NP_217904.1 transposase -
Rv3388 3801653..3803848 + 2196 YP_177968.1 PE-PGRS family protein PE_PGRS52 -
Rv3389c 3803919..3804791 - 873 NP_217906.1 3-hydroxyacyl-thioester dehydratase HtdY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside IScluster/Tn - - 3795100..3801463 6363


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(12-64)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14316.74 Da        Isoelectric Point: 10.7249

>T131 NP_217901.1 NC_000962:c3799635-3799243 [Mycobacterium tuberculosis H37Rv]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Download         Length: 393 bp

>T131 NC_000962:c3799635-3799243 [Mycobacterium tuberculosis H37Rv]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11030.59 Da        Isoelectric Point: 4.5332

>AT131 NP_217902.1 NC_000962:c3799943-3799635 [Mycobacterium tuberculosis H37Rv]
MTPTACATVSTMTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLPEP
LDLDAGVELPSVTLARLREHER

Download         Length: 309 bp

>AT131 NC_000962:c3799943-3799635 [Mycobacterium tuberculosis H37Rv]
ATGACGCCGACCGCTTGTGCTACAGTTAGCACAATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCT
GCTGCGCCGAGTCGAGGCCGGCGAGACGATCGAGATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGC
AGGGCGGTCCATACGAGCAGCTGCTGGCAAGCGGCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCG
CTCGACCTCGACGCGGGCGTCGAGCTGCCGTCGGTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6245 Mycobacterium tuberculosis H37Rv

43.077

100

0.431
T132 Mycobacterium tuberculosis H37Rv

35.115

100

0.354

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT77 Mycobacterium tuberculosis H37Rv

39.437

83.529

0.329

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB O50411

Antitoxin

Source ID Structure
AlphaFold DB P9WF13

References

(1) Madhurima Roy et al. (2020) Biochemical Characterization of VapC46 Toxin from Mycobacterium tuberculosis. Molecular Biotechnology 62(6-7):335-343. [PubMed:32236842] experimental

(2) Madhurima Roy et al. (2019) Structural characterization of VapB46 antitoxin from Mycobacterium tuberculosis: insights into VapB46-DNA binding. The FEBS Journal 286(6):1174-1190. [PubMed:30576065] experimental

(3) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113] experimental


experimental experimental literature