6245

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2204866..2206802 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name vapC Uniprot ID P9WF67
Locus tag Rv1962c Protein ID NP_216478.1
Coordinates 2204866..2205273 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95251
Locus tag Rv1963c Protein ID NP_216479.1
Coordinates 2205582..2206802 (-) Length 407 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv1950c 2199998..2200189 - 192 NP_216466.1 hypothetical protein -
Rv1951c 2200190..2200486 - 297 NP_216467.1 hypothetical protein -
Rv1952 2200726..2200941 + 216 NP_216468.1 antitoxin VapB14 -
Rv1953 2200938..2201249 + 312 NP_216469.1 ribonuclease VapC14 -
Rv1954c 2201223..2201744 - 522 NP_216470.1 hypothetical protein -
Rv1955 2201719..2202096 + 378 NP_216471.2 toxin HigB -
Rv1956 2202138..2202587 + 450 NP_216472.1 antitoxin HigA -
Rv1957 2202584..2203129 + 546 NP_216473.1 SecB-like chaperone -
Rv1958c 2203018..2203632 - 615 NP_216474.1 hypothetical protein -
Rv1959c 2203681..2203977 - 297 NP_216475.1 toxin ParE1 -
Rv1960c 2203974..2204225 - 252 NP_216476.1 antitoxin ParD1 -
Rv1961 2204212..2204706 + 495 NP_216477.1 hypothetical protein -
Rv1962c 2204866..2205273 - 408 NP_216478.1 ribonuclease VapC35 Toxin
Rv1962A 2205277..2205549 - 273 YP_007410641.1 antitoxin VapB35 -
Rv1963c 2205582..2206802 - 1221 NP_216479.1 transcriptional repressor Mce3R Antitoxin
Rv1964 2207700..2208497 + 798 NP_216480.1 integral membrane protein -
Rv1965 2208507..2209322 + 816 NP_216481.1 integral membrane protein -
Rv1966 2209327..2210604 + 1278 YP_177852.1 Mce family protein Mce3A -
Rv1967 2210601..2211629 + 1029 NP_216483.1 Mce family protein Mce3B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-122)

Antitoxin

DNA-binding HTH domain, TetR-type

(223-269)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14488.60 Da        Isoelectric Point: 4.5732

>T6245 NP_216478.1 NC_000962:c2205273-2204866 [Mycobacterium tuberculosis H37Rv]
VIYLETSALVKLIRIEVESDALADWLDDRTELRWITSALTEVELSRAIRAVSPEGLPAVPSVLARLDRFEIDAVIRSTAA
AYPNPALRSLDAIHLATAQTAGSVAPLTALVTYDNRLKEAAEALSLAVVAPGQAR

Download         Length: 408 bp

>T6245 NC_000962:c2205273-2204866 [Mycobacterium tuberculosis H37Rv]
GTGATCTACCTGGAAACCTCGGCGCTGGTCAAGCTAATTCGGATCGAGGTCGAATCTGACGCTTTAGCTGATTGGCTCGA
TGACCGCACCGAACTACGGTGGATCACATCGGCTTTGACCGAAGTTGAGCTATCCAGAGCCATCCGCGCAGTCTCCCCAG
AAGGGCTACCGGCGGTGCCCTCCGTGCTAGCCAGGCTGGACCGTTTCGAAATCGACGCGGTCATACGCTCCACCGCGGCG
GCCTACCCCAACCCAGCACTGCGCTCGCTCGACGCGATACACCTAGCCACTGCTCAAACCGCCGGATCCGTTGCACCATT
GACCGCGCTGGTTACCTACGACAACCGCCTTAAGGAAGCCGCCGAGGCCCTGTCGTTGGCGGTCGTCGCACCCGGACAAG
CGCGATGA

Antitoxin


Download         Length: 407 a.a.        Molecular weight: 44341.66 Da        Isoelectric Point: 8.7527

>AT6245 NP_216479.1 NC_000962:c2206802-2205582 [Mycobacterium tuberculosis H37Rv]
MASVAQPVRRRPKDRKKQILDQAVGLFIERGFHSVKLEDIAEAAGVTARALYRHYDNKQALLAEAIRTGQDQYQSARRLT
EGETEPTPRPLNADLEDLIAAAVASRALTVLWQREARYLNEDDRTAVRRRINAIVAGMRDSVLLEVPDLSPQHSELRAWA
VSSTLTSLGRHSLSLPGEELKKLLYQACMAAARTPPVCELPPLPAGDAARDEADVLFSRYETLLAAGARLFRAQGYPAVN
TSEIGKGAGIAGPGLYRSFSSKQAILDALIRRLDEWRCLECIRALRANQQAAQRLRGLVQGHVRISLDAPDLVAVSVTEL
SHASVEVRDGYLRNQGDREAVWIDLIGKLVPATSVAQGRLLVAAAISFIEDVARTWHLTRYAGVADEISGLALAILTSGA
GNLLRA

Download         Length: 1221 bp

>AT6245 NC_000962:c2206802-2205582 [Mycobacterium tuberculosis H37Rv]
ATGGCATCCGTCGCCCAACCCGTTAGGCGCCGCCCAAAGGACCGGAAGAAGCAGATTTTGGATCAGGCCGTTGGACTGTT
CATCGAACGTGGCTTCCATTCGGTCAAATTGGAGGACATTGCCGAGGCGGCCGGGGTGACCGCGCGCGCGTTGTATCGCC
ACTACGACAACAAGCAGGCGTTGCTCGCCGAAGCGATCCGAACCGGCCAGGATCAGTACCAGAGCGCGCGTCGTCTCACC
GAGGGCGAGACGGAGCCGACGCCGCGGCCGTTGAACGCCGATCTGGAAGACCTGATCGCCGCGGCGGTCGCCTCTCGGGC
GTTGACGGTGCTGTGGCAGCGCGAGGCCCGCTACCTCAACGAGGACGACCGCACGGCGGTCCGGCGCCGCATCAACGCGA
TCGTCGCCGGCATGCGTGACAGCGTGCTGCTGGAGGTGCCCGATCTGAGTCCACAGCATTCGGAGTTGCGGGCGTGGGCG
GTGTCCAGCACTTTGACCAGCCTGGGCCGGCACAGCCTAAGCCTGCCGGGCGAGGAACTGAAAAAGCTTCTCTACCAGGC
GTGTATGGCCGCGGCAAGGACGCCTCCCGTCTGCGAATTGCCGCCACTGCCGGCCGGTGATGCCGCACGCGACGAGGCCG
ACGTGCTGTTCTCCCGCTACGAGACCCTGCTGGCCGCGGGCGCGCGGCTGTTCCGTGCGCAGGGCTATCCGGCCGTCAAC
ACCAGCGAAATCGGCAAGGGAGCCGGCATCGCGGGCCCGGGGCTGTACCGTTCGTTTTCTTCCAAACAGGCCATCCTGGA
CGCGCTCATCCGCCGCCTCGACGAGTGGCGCTGCCTGGAGTGCATCCGAGCGCTACGAGCGAATCAGCAAGCGGCACAAC
GGTTGCGCGGCCTTGTCCAAGGGCACGTTCGGATCAGCTTGGACGCTCCGGATCTGGTGGCAGTGTCGGTCACCGAACTG
TCGCACGCCTCTGTCGAAGTACGCGACGGCTACCTGCGAAATCAGGGCGACCGCGAGGCCGTGTGGATCGACCTCATCGG
CAAGCTGGTACCCGCGACCAGTGTCGCCCAGGGGCGACTGCTGGTCGCGGCGGCGATTAGCTTCATCGAAGACGTCGCTC
GCACCTGGCATCTCACGCGCTACGCCGGAGTCGCCGACGAGATCAGTGGCCTGGCGCTGGCGATCCTGACCAGCGGGGCA
GGTAACCTCTTGCGCGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T131 Mycobacterium tuberculosis H37Rv

43.077

100

0.431
T132 Mycobacterium tuberculosis H37Rv

36.17

100

0.378

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P9WF67

Antitoxin

Source ID Structure
AlphaFold DB P95251

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113] experimental


experimental experimental literature