Detailed information of TA system TA000132

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PHD-PIN
• Location: 3826252..3826958
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF49
• Locus tag: Rv3408
• Protein ID: NP_217925.1
• Coordinates: 3826548..3826958 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WF23
• Locus tag: Rv3407
• Protein ID: NP_217924.1
• Coordinates: 3826252..3826551 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv3403c	3822262..3823863	-	1602	NP_217920.1	hypothetical protein	-
Rv3404c	3823880..3824584	-	705	NP_217921.1	hypothetical protein	-
Rv3405c	3824702..3825268	-	567	NP_217922.1	HTH-type transcriptional regulator	-
Rv3406	3825330..3826217	+	888	NP_217923.1	dioxygenase	-
Rv3407	3826252..3826551	+	300	NP_217924.1	antitoxin VapB47	Antitoxin
Rv3408	3826548..3826958	+	411	NP_217925.1	ribonuclease VapC47	Toxin
Rv3409c	3826991..3828727	-	1737	NP_217926.1	cholesterol oxidase	-
Rv3410c	3828783..3829910	-	1128	NP_217927.1	oxidoreductase	-
Rv3411c	3829930..3831519	-	1590	NP_217928.1	inosine-5'-monophosphate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-61)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14669.76 Da
• Isoelectric Point: 5.1784

>T132 NP_217925.1 NC_000962:3826548-3826958 [Mycobacterium tuberculosis H37Rv]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVESMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T132 NC_000962:3826548-3826958 [Mycobacterium tuberculosis H37Rv]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTCGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11009.66 Da
• Isoelectric Point: 10.7399

>AT132 NP_217924.1 NC_000962:3826252-3826551 [Mycobacterium tuberculosis H37Rv]
MRATVGLVEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEP
ARGRKRTLSDVLNEMRDEQ

Nucleotide

• Length: 300 bp

>AT132 NC_000962:3826252-3826551 [Mycobacterium tuberculosis H37Rv]
ATGCGTGCTACCGTTGGGCTTGTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGT
TGAAGCCGGCGAGGAACTTGGCGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTT
CTCGCGAAGCCCTGATTGAATCAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCG
GCGCGCGGCCGCAAGCGCACCCTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6245	Mycobacterium tuberculosis H37Rv	36.17	100	0.378
T131	Mycobacterium tuberculosis H37Rv	35.115	100	0.354

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P9WF49

Antitoxin

• AlphaFold DB: P9WF23

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]


 experimental literature