Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/PHD-PIN |
| Location | 694839..695485 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | O07783 |
| Locus tag | Rv0595c | Protein ID | NP_215109.1 |
| Coordinates | 694839..695231 (-) | Length | 131 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | P9WF21 |
| Locus tag | Rv0596c | Protein ID | NP_215110.1 |
| Coordinates | 695228..695485 (-) | Length | 86 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv0592 | 690501..692027 | + | 1527 | NP_215106.1 | Mce family protein Mce2D | - |
| Rv0593 | 692024..693232 | + | 1209 | NP_215107.1 | Mce family lipoprotein LprL | - |
| Rv0594 | 693237..694787 | + | 1551 | NP_215108.1 | Mce family protein Mce2F | - |
| Rv0595c | 694839..695231 | - | 393 | NP_215109.1 | ribonuclease VapC4 | Toxin |
| Rv0596c | 695228..695485 | - | 258 | NP_215110.1 | antitoxin VapB4 | Antitoxin |
| Rv0597c | 695668..696903 | - | 1236 | NP_215111.1 | hypothetical protein | - |
| Rv0598c | 697154..697567 | - | 414 | NP_215112.1 | ribonuclease VapC27 | - |
| Rv0599c | 697564..697800 | - | 237 | NP_215113.1 | antitoxin VapB27 | - |
| Rv0600c | 697904..698410 | - | 507 | NP_215114.2 | two component sensor kinase HK1 | - |
| Rv0601c | 698524..698994 | - | 471 | NP_215115.1 | two component sensor kinase HK2 | - |
| Rv0602c | 699038..699799 | - | 762 | NP_215116.1 | two component DNA binding transcriptional regulator TcrA | - |
| Rv0603 | 699856..700167 | + | 312 | NP_215117.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 131 a.a. Molecular weight: 14080.08 Da Isoelectric Point: 4.6558
>T77 NP_215109.1 NC_000962:c695231-694839 [Mycobacterium tuberculosis H37Rv]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI
Download Length: 393 bp
>T77 NC_000962:c695231-694839 [Mycobacterium tuberculosis H37Rv]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA
Antitoxin
Download Length: 86 a.a. Molecular weight: 9681.06 Da Isoelectric Point: 7.0175
>AT77 NP_215110.1 NC_000962:c695485-695228 [Mycobacterium tuberculosis H37Rv]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW
Download Length: 258 bp
>AT77 NC_000962:c695485-695228 [Mycobacterium tuberculosis H37Rv]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T82 | Mycobacterium tuberculosis H37Rv |
40.769 |
100 |
0.408 |
| T86 | Mycobacterium tuberculosis H37Rv |
42.308 |
92.857 |
0.393 |
| T6113 | Synechocystis sp. PCC 6803 |
30 |
100 |
0.3 |
| T107 | Mycobacterium tuberculosis H37Rv |
31.2 |
96.154 |
0.3 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT131 | Mycobacterium tuberculosis H37Rv |
39.437 |
83.529 |
0.329 |
| AT82 | Mycobacterium tuberculosis H37Rv |
47.273 |
64.706 |
0.306 |
Multiple sequence alignment
References
(1) Sakshi Agarwal et al. (2018) System-Wide Analysis Unravels the Differential Regulation and In Vivo Essentiality of Virulence-Associated Proteins B and C Toxin-Antitoxin Systems of Mycobacterium tuberculosis. The Journal of Infectious Diseases 217(11):1809-1820. [PubMed:29529224]
(2) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]
(3) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878]
(4) Jared D Sharp et al. (2012) Growth and translation inhibition through sequence-specific RNA binding by Mycobacterium tuberculosis VapC toxin. The Journal of Biological Chemistry 287(16):12835-47. [PubMed:22354968]
(5) Valdir C Barth et al. (2021) Mycobacterium tuberculosis VapC4 toxin engages small ORFs to initiate an integrated oxidative and copper stress response. Proceedings of The National Academy of Sciences of The United States of America 118(32):e2022136118. [PubMed:34362841]