Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/PHD-PIN |
| Location | 718025..718689 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | P96917 |
| Locus tag | Rv0627 | Protein ID | NP_215141.1 |
| Coordinates | 718282..718689 (+) | Length | 136 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | P9WF19 |
| Locus tag | Rv0626 | Protein ID | NP_215140.1 |
| Coordinates | 718025..718285 (+) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv0621 | 714202..715266 | + | 1065 | NP_215135.1 | membrane protein | - |
| Rv0622 | 715370..716317 | + | 948 | NP_215136.2 | membrane protein | - |
| Rv0623 | 716410..716664 | + | 255 | NP_215137.1 | antitoxin VapB30 | - |
| Rv0624 | 716664..717059 | + | 396 | NP_215138.1 | ribonuclease VapC30 | - |
| Rv0625c | 717153..717893 | - | 741 | NP_215139.1 | transmembrane protein | - |
| Rv0626 | 718025..718285 | + | 261 | NP_215140.1 | antitoxin VapB5 | Antitoxin |
| Rv0627 | 718282..718689 | + | 408 | NP_215141.1 | ribonuclease VapC5 | Toxin |
| Rv0628c | 718761..719912 | - | 1152 | NP_215142.1 | hypothetical protein | - |
| Rv0629c | 720005..721732 | - | 1728 | NP_215143.1 | exonuclease V subunit alpha RecD | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 136 a.a. Molecular weight: 14376.37 Da Isoelectric Point: 4.3568
>T82 NP_215141.1 NC_000962:718282-718689 [Mycobacterium tuberculosis H37Rv]
VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAAR
MWARLRIHLAESGRRVRINDLWIAAVAASRALPVITQDDDFAALDGAASVEIIRV
VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAAR
MWARLRIHLAESGRRVRINDLWIAAVAASRALPVITQDDDFAALDGAASVEIIRV
Download Length: 408 bp
>T82 NC_000962:718282-718689 [Mycobacterium tuberculosis H37Rv]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA
Antitoxin
Download Length: 87 a.a. Molecular weight: 9492.84 Da Isoelectric Point: 11.9108
>AT82 NP_215140.1 NC_000962:718025-718285 [Mycobacterium tuberculosis H37Rv]
MSEVASRELRNDTAGVLRRVRAGEDVTITVSGRPVAVLTPVRPRRRRWLSKTEFLSRLRGAQADPGLRNDLAVLAGDTTE
DLGPIR
MSEVASRELRNDTAGVLRRVRAGEDVTITVSGRPVAVLTPVRPRRRRWLSKTEFLSRLRGAQADPGLRNDLAVLAGDTTE
DLGPIR
Download Length: 261 bp
>AT82 NC_000962:718025-718285 [Mycobacterium tuberculosis H37Rv]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T77 | Mycobacterium tuberculosis H37Rv |
40.769 |
100 |
0.408 |
| T86 | Mycobacterium tuberculosis H37Rv |
39.216 |
91.071 |
0.357 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT77 | Mycobacterium tuberculosis H37Rv |
47.273 |
64.706 |
0.306 |
Multiple sequence alignment
References
(1) Linda Miallau et al. (2009) Structure and proposed activity of a member of the VapBC family of toxin-antitoxin systems. VapBC-5 from Mycobacterium tuberculosis. The Journal of Biological Chemistry 284(1):276-283. [PubMed:18952600]
(2) Uddipan Das et al. (2013) Preliminary crystallographic analysis of recombinant VapBC-15 toxin-antitoxin complex from Mycobacterium tuberculosis. Acta Crystallographica. Section F, Structural Biology And Crystallization Communications 69(Pt 11):1242-5. [PubMed:24192359]