Detailed information of TA system TA000086

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 758532..759139
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB1
• Locus tag: Rv0665
• Protein ID: NP_215179.1
• Coordinates: 758801..759139 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O06775
• Locus tag: Rv0664
• Protein ID: NP_215178.1
• Coordinates: 758532..758804 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv0658c	753693..754409	-	717	NP_215172.1	integral membrane protein	-
Rv0659c	754685..754993	-	309	NP_215173.1	toxin MazF2	-
Rv0660c	754980..755225	-	246	NP_215174.1	antitoxin MazE2	-
Rv0661c	755335..755772	-	438	NP_215175.1	ribonuclease VapC7	-
Rv0662c	755769..756023	-	255	NP_215176.2	antitoxin VapB7	-
Rv0663	756137..758500	+	2364	NP_215177.1	arylsulfatase AtsD	-
Rv0664	758532..758804	+	273	NP_215178.1	antitoxin VapB8	Antitoxin
Rv0665	758801..759139	+	339	NP_215179.1	ribonuclease VapC8	Toxin
Rv0666	759136..759309	+	174	NP_215180.1	membrane protein	-
Rv0667	759807..763325	+	3519	NP_215181.1	DNA-directed RNA polymerase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 11558.34 Da
• Isoelectric Point: 4.8011

>T86 NP_215179.1 NC_000962:758801-759139 [Mycobacterium tuberculosis H37Rv]
VTEGEVGVGLLDTSVFIARESGGAIADLPERVALSVMTIGELQLGLLNAGDSATRSRRADTLALARTADQIPVSEAVMIS
LARLVADCRAAGVRRSVKLTDALIAATAEIKV

Nucleotide

• Length: 339 bp

>T86 NC_000962:758801-759139 [Mycobacterium tuberculosis H37Rv]
GTGACCGAGGGCGAGGTCGGGGTAGGCCTGCTAGATACGTCGGTCTTCATTGCGCGCGAGAGCGGCGGTGCAATCGCGGA
CCTGCCTGAACGCGTGGCGCTTTCGGTTATGACGATCGGTGAGCTGCAACTCGGTCTGCTCAATGCTGGCGATTCGGCGA
CCCGATCACGACGCGCCGACACCCTCGCGCTAGCGCGCACGGCCGATCAGATCCCTGTCAGTGAAGCGGTGATGATTTCG
TTGGCTCGACTCGTCGCGGACTGCCGAGCCGCGGGCGTGCGGCGGTCGGTGAAGCTGACCGACGCTCTCATTGCGGCAAC
CGCGGAGATCAAGGTGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9250.20 Da
• Isoelectric Point: 9.4208

>AT86 NP_215178.1 NC_000962:758532-758804 [Mycobacterium tuberculosis H37Rv]
MEKSRCHAVAHGGGCAGSAKSHKSGGRCGQGRGAGDSHGTRGAGRRYRAASAPHPLAVGAHLRDELAKRSADPRLTDELN
DLAGHTLDDL

Nucleotide

• Length: 273 bp

>AT86 NC_000962:758532-758804 [Mycobacterium tuberculosis H37Rv]
ATGGAAAAGTCACGGTGCCACGCTGTCGCACATGGAGGTGGGTGTGCGGGATCTGCGAAATCGCACAAGTCAGGTGGTCG
ATGCGGTCAAGGCCGGGGTGCCGGTGACTCTCACGGTACACGGGGAGCCGGTCGCCGATATCGTGCCGCATCGGCGCCGC
ATCCGCTGGCTGTCGGGGCGCATCTGCGCGATGAGCTCGCCAAGCGCTCGGCCGACCCGCGCCTCACCGATGAACTCAAC
GACTTGGCCGGTCATACCCTCGACGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T77	Mycobacterium tuberculosis H37Rv	42.308	92.857	0.393
T82	Mycobacterium tuberculosis H37Rv	39.216	91.071	0.357

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: P9WFB1

Antitoxin

• AlphaFold DB: O06775

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]


 experimental literature