Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/RHH-PIN |
| Location | 1399..2032 | Replicon | plasmid pSYSA_M |
| Accession | NC_020287 | ||
| Organism | Synechocystis sp. PCC 6803 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | MYO_RS17195 | Protein ID | WP_011153672.1 |
| Coordinates | 1399..1827 (-) | Length | 143 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | - |
| Locus tag | MYO_RS17200 | Protein ID | WP_011153673.1 |
| Coordinates | 1799..2032 (-) | Length | 78 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| MYO_RS17190 (MYO_430) | 706..1287 | - | 582 | WP_011153671.1 | IS701 family transposase | - |
| MYO_RS17195 (MYO_440) | 1399..1827 | - | 429 | WP_011153672.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| MYO_RS17200 (MYO_450) | 1799..2032 | - | 234 | WP_011153673.1 | DNA-binding protein | Antitoxin |
| MYO_RS17205 (MYO_460) | 2399..2965 | + | 567 | WP_011153674.1 | site-specific integrase | - |
| MYO_RS17210 (MYO_470) | 3041..3364 | - | 324 | WP_011153675.1 | toxin-antitoxin system HicB family antitoxin | - |
| MYO_RS17215 | 3367..3819 | - | 453 | WP_048908403.1 | putative toxin-antitoxin system toxin component, PIN family | - |
| MYO_RS17220 (MYO_490) | 4241..5257 | + | 1017 | WP_011153677.1 | IS4-like element IS4Sa family transposase | - |
| MYO_RS17225 (MYO_4100) | 5340..6284 | - | 945 | WP_011153678.1 | WYL domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| inside | Non-Mobilizable plasmid | - | - | 1..100749 | 100749 | ||
| - | flank | IS/Tn | - | - | 4241..5257 | 1016 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 143 a.a. Molecular weight: 15986.31 Da Isoelectric Point: 5.1630
>T6113 WP_011153672.1 NC_020287:c1827-1399 [Synechocystis sp. PCC 6803]
MTEPVKYLIDTNVISELRKQDRADPGVLAFFQQAQHLYLSVITIGELRRGVELIRHRGDVQQANLLEEWLEIVIADYADH
ILDFTIFEAQVLGKLRVPNPHNALDKQIAATALSYGLTLVTRNVKDFADTGVCLLNPFTGNQ
MTEPVKYLIDTNVISELRKQDRADPGVLAFFQQAQHLYLSVITIGELRRGVELIRHRGDVQQANLLEEWLEIVIADYADH
ILDFTIFEAQVLGKLRVPNPHNALDKQIAATALSYGLTLVTRNVKDFADTGVCLLNPFTGNQ
Download Length: 429 bp
>T6113 NC_020287:c1827-1399 [Synechocystis sp. PCC 6803]
ATGACAGAGCCAGTCAAATATTTGATTGATACCAACGTTATTAGTGAGCTACGGAAACAAGATAGGGCCGACCCCGGTGT
ACTAGCATTTTTTCAGCAAGCTCAGCATCTCTATTTGAGTGTGATTACCATTGGGGAATTGCGGCGGGGTGTGGAATTAA
TCAGGCATCGGGGAGATGTTCAACAAGCCAATTTACTGGAGGAGTGGTTGGAAATTGTAATTGCTGATTACGCTGACCAC
ATCCTAGATTTCACCATCTTCGAGGCTCAAGTTTTGGGAAAGTTGCGGGTTCCTAATCCCCATAACGCCCTAGATAAACA
AATTGCCGCCACTGCCCTTAGTTATGGACTGACCCTAGTTACTCGCAATGTCAAGGATTTTGCCGACACAGGAGTTTGTT
TGCTTAATCCTTTCACCGGCAATCAATAA
ATGACAGAGCCAGTCAAATATTTGATTGATACCAACGTTATTAGTGAGCTACGGAAACAAGATAGGGCCGACCCCGGTGT
ACTAGCATTTTTTCAGCAAGCTCAGCATCTCTATTTGAGTGTGATTACCATTGGGGAATTGCGGCGGGGTGTGGAATTAA
TCAGGCATCGGGGAGATGTTCAACAAGCCAATTTACTGGAGGAGTGGTTGGAAATTGTAATTGCTGATTACGCTGACCAC
ATCCTAGATTTCACCATCTTCGAGGCTCAAGTTTTGGGAAAGTTGCGGGTTCCTAATCCCCATAACGCCCTAGATAAACA
AATTGCCGCCACTGCCCTTAGTTATGGACTGACCCTAGTTACTCGCAATGTCAAGGATTTTGCCGACACAGGAGTTTGTT
TGCTTAATCCTTTCACCGGCAATCAATAA
Antitoxin
Download Length: 78 a.a. Molecular weight: 8731.99 Da Isoelectric Point: 7.2049
>AT6113 WP_011153673.1 NC_020287:c2032-1799 [Synechocystis sp. PCC 6803]
MANLIVRNIDEAVVVALKKRAGQHGISAEAEHRKILEQALLRLPRKPFAEVLRQIPNVGNDSDFERIQDDRASQIFD
MANLIVRNIDEAVVVALKKRAGQHGISAEAEHRKILEQALLRLPRKPFAEVLRQIPNVGNDSDFERIQDDRASQIFD
Download Length: 234 bp
>AT6113 NC_020287:c2032-1799 [Synechocystis sp. PCC 6803]
ATGGCTAATTTAATTGTTCGCAATATTGATGAAGCGGTGGTGGTGGCCCTGAAAAAGCGGGCGGGGCAACATGGCATTAG
TGCCGAAGCAGAACATCGCAAAATCCTAGAGCAGGCCCTATTACGACTTCCCCGAAAGCCCTTTGCCGAGGTGCTCCGCC
AGATTCCCAATGTGGGCAATGACTCAGATTTTGAGCGAATACAGGATGACAGAGCCAGTCAAATATTTGATTGA
ATGGCTAATTTAATTGTTCGCAATATTGATGAAGCGGTGGTGGTGGCCCTGAAAAAGCGGGCGGGGCAACATGGCATTAG
TGCCGAAGCAGAACATCGCAAAATCCTAGAGCAGGCCCTATTACGACTTCCCCGAAAGCCCTTTGCCGAGGTGCTCCGCC
AGATTCCCAATGTGGGCAATGACTCAGATTTTGAGCGAATACAGGATGACAGAGCCAGTCAAATATTTGATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10034 | Thermus thermophilus HB27 |
41.844 |
100 |
0.428 |
| T843 | Bradyrhizobium diazoefficiens USDA 110 |
38.732 |
100 |
0.39 |
| T10138 | Pseudomonas savastanoi |
35 |
100 |
0.353 |
| T4923 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
35.461 |
99.296 |
0.352 |
| T1070 | Haemophilus influenzae Rd KW20 |
32.558 |
97.727 |
0.318 |
| T6112 | uncultured bacterium |
28.873 |
100 |
0.308 |
| T10054 | Bosea sp. PAMC 26642 strain PAMC26642 |
31.008 |
97.727 |
0.303 |
| T77 | Mycobacterium tuberculosis H37Rv |
30 |
100 |
0.3 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10033 | Thermus thermophilus HB27 |
37.063 |
100 |
0.384 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Stefan Kopfmann et al. (2013) Toxin-antitoxin systems on the large defense plasmid pSYSA of Synechocystis sp. PCC 6803. The Journal of Biological Chemistry 288(10):7399-409. [PubMed:23322786]