Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/- |
| Location | 145383..146056 | Replicon | plasmid pTT27 |
| Accession | NC_005838 | ||
| Organism | Thermus thermophilus HB27 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | TT_RS10840 | Protein ID | WP_011174422.1 |
| Coordinates | 145383..145799 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | - |
| Locus tag | TT_RS10845 | Protein ID | WP_041443928.1 |
| Coordinates | 145796..146056 (-) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| TT_RS11680 | 143305..143705 | + | 401 | Protein_139 | IS630 family transposase | - |
| TT_RS10835 (TT_P0140) | 143693..144850 | + | 1158 | WP_148187591.1 | ATP-binding protein | - |
| TT_RS11790 | 145011..145148 | + | 138 | WP_162467451.1 | hypothetical protein | - |
| TT_RS10840 (TT_P0142) | 145383..145799 | - | 417 | WP_011174422.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| TT_RS10845 (TT_P0143) | 145796..146056 | - | 261 | WP_041443928.1 | type II toxin-antitoxin system prevent-host-death family antitoxin | Antitoxin |
| TT_RS11695 | 146269..146613 | - | 345 | Protein_144 | hypothetical protein | - |
| TT_RS10855 (TT_P0146) | 148386..149594 | + | 1209 | WP_041444003.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..232605 | 232605 | |
| flank | IS/Tn | - | - | 143389..143634 | 245 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15274.81 Da Isoelectric Point: 8.6238
>T10034 WP_011174422.1 NC_005838:c145799-145383 [Thermus thermophilus HB27]
MSYLLDTNVVSEAAKQRPHPKVLAWLSETPLGQAYLSALTLGELVQGVVRAPEARKPGLERWLEGLKQRFAGRILPLDAE
VMETWGLLTGEALREGRPLSPLDALLAATALRHGLVLVTRNVAHFRGLPLRLLNPWEE
MSYLLDTNVVSEAAKQRPHPKVLAWLSETPLGQAYLSALTLGELVQGVVRAPEARKPGLERWLEGLKQRFAGRILPLDAE
VMETWGLLTGEALREGRPLSPLDALLAATALRHGLVLVTRNVAHFRGLPLRLLNPWEE
Download Length: 417 bp
>T10034 NC_005838:c145799-145383 [Thermus thermophilus HB27]
TTGAGCTACCTCCTGGACACCAACGTGGTGTCCGAGGCCGCCAAGCAAAGGCCTCATCCCAAGGTTCTGGCCTGGCTCTC
AGAGACCCCTCTGGGACAGGCCTACCTCTCCGCCCTCACCCTGGGGGAACTGGTCCAGGGGGTGGTCCGGGCTCCCGAGG
CCCGGAAGCCGGGGCTGGAGCGGTGGCTGGAGGGGCTCAAGCAGCGCTTTGCGGGGAGGATCCTGCCTCTGGACGCGGAG
GTCATGGAAACTTGGGGTCTCCTCACAGGGGAGGCCCTCCGGGAGGGACGTCCCCTTTCCCCTCTGGATGCCCTGCTCGC
CGCCACCGCTCTCCGCCACGGCCTCGTCCTGGTCACCCGGAACGTGGCCCATTTCCGGGGTCTCCCCCTACGGTTGCTCA
ATCCCTGGGAGGAATAG
TTGAGCTACCTCCTGGACACCAACGTGGTGTCCGAGGCCGCCAAGCAAAGGCCTCATCCCAAGGTTCTGGCCTGGCTCTC
AGAGACCCCTCTGGGACAGGCCTACCTCTCCGCCCTCACCCTGGGGGAACTGGTCCAGGGGGTGGTCCGGGCTCCCGAGG
CCCGGAAGCCGGGGCTGGAGCGGTGGCTGGAGGGGCTCAAGCAGCGCTTTGCGGGGAGGATCCTGCCTCTGGACGCGGAG
GTCATGGAAACTTGGGGTCTCCTCACAGGGGAGGCCCTCCGGGAGGGACGTCCCCTTTCCCCTCTGGATGCCCTGCTCGC
CGCCACCGCTCTCCGCCACGGCCTCGTCCTGGTCACCCGGAACGTGGCCCATTTCCGGGGTCTCCCCCTACGGTTGCTCA
ATCCCTGGGAGGAATAG
Antitoxin
Download Length: 87 a.a. Molecular weight: 9818.05 Da Isoelectric Point: 4.9126
>AT10034 WP_041443928.1 NC_005838:c146056-145796 [Thermus thermophilus HB27]
VGRVWQLQEAKARFSEVVERALKGEPQTVTRRGRPVVVVVDWATYTRLRGEEASLWEALRAPEPLSEEEAETLFTREAAG
YREVGF
VGRVWQLQEAKARFSEVVERALKGEPQTVTRRGRPVVVVVDWATYTRLRGEEASLWEALRAPEPLSEEEAETLFTREAAG
YREVGF
Download Length: 261 bp
>AT10034 NC_005838:c146056-145796 [Thermus thermophilus HB27]
GTGGGACGGGTGTGGCAACTCCAAGAAGCCAAAGCCCGCTTCTCCGAAGTGGTGGAGCGGGCTCTTAAGGGTGAACCCCA
GACCGTTACCCGGCGGGGGCGGCCCGTGGTGGTGGTGGTGGACTGGGCTACCTACACCCGCCTCCGGGGGGAGGAGGCCT
CCCTTTGGGAGGCTCTCCGTGCCCCTGAGCCCCTTTCCGAGGAGGAGGCGGAAACCCTTTTCACCCGGGAGGCTGCGGGG
TACCGGGAGGTGGGGTTTTGA
GTGGGACGGGTGTGGCAACTCCAAGAAGCCAAAGCCCGCTTCTCCGAAGTGGTGGAGCGGGCTCTTAAGGGTGAACCCCA
GACCGTTACCCGGCGGGGGCGGCCCGTGGTGGTGGTGGTGGACTGGGCTACCTACACCCGCCTCCGGGGGGAGGAGGCCT
CCCTTTGGGAGGCTCTCCGTGCCCCTGAGCCCCTTTCCGAGGAGGAGGCGGAAACCCTTTTCACCCGGGAGGCTGCGGGG
TACCGGGAGGTGGGGTTTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10033 | Thermus thermophilus HB27 |
70 |
93.023 |
0.651 |
| T843 | Bradyrhizobium diazoefficiens USDA 110 |
44.286 |
100 |
0.449 |
| T6113 | Synechocystis sp. PCC 6803 |
41.844 |
100 |
0.428 |
| T10138 | Pseudomonas savastanoi |
35.294 |
98.551 |
0.348 |
| T4923 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
31.25 |
100 |
0.326 |
| T10054 | Bosea sp. PAMC 26642 strain PAMC26642 |
31.387 |
100 |
0.326 |
| T4914 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
30.37 |
100 |
0.318 |
| T1070 | Haemophilus influenzae Rd KW20 |
29.197 |
100 |
0.303 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10033 | Thermus thermophilus HB27 |
73.723 |
99.275 |
0.732 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Haijuan Li et al. (2023) The parABSm system is involved in megaplasmid partitioning and genome integrity maintenance in Thermus thermophilus. G3 (Bethesda, Md.) 13(4):jkad038. [PubMed:36786449]