Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/AbrB-PIN |
| Location | 722007..722657 | Replicon | chromosome |
| Accession | NC_010278 | ||
| Organism | Actinobacillus pleuropneumoniae serovar 3 str. JL03 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | B0BNU3 |
| Locus tag | APJL_0659 | Protein ID | WP_005614926.1 |
| Coordinates | 722007..722396 (-) | Length | 130 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | B0BNU4 |
| Locus tag | APJL_0660 | Protein ID | WP_005604117.1 |
| Coordinates | 722397..722657 (-) | Length | 87 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| APJL_RS03215 (APJL_0657) | 717247..720522 | - | 3276 | WP_012262906.1 | DEAD/DEAH box helicase family protein | - |
| APJL_RS03220 (APJL_0658) | 720648..721871 | - | 1224 | WP_012262907.1 | ATP-binding protein | - |
| APJL_RS03225 (APJL_0659) | 722007..722396 | - | 390 | WP_005614926.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| APJL_RS03230 (APJL_0660) | 722397..722657 | - | 261 | WP_005604117.1 | hypothetical protein | Antitoxin |
| APJL_RS03235 (APJL_0661) | 723003..723839 | + | 837 | WP_012262908.1 | hypothetical protein | - |
| APJL_RS03240 (APJL_0662) | 723871..725892 | - | 2022 | WP_012262909.1 | excinuclease ABC subunit B | - |
| APJL_RS03245 (APJL_0663) | 726069..726881 | + | 813 | WP_005607533.1 | EfeM/EfeO family lipoprotein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 130 a.a. Molecular weight: 14837.17 Da Isoelectric Point: 5.1295
>T4914 WP_005614926.1 NC_010278:c722396-722007 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK
MYMLDTNTVSYFFRQDPTVVKKLQQLNPELICISSVTAAELFYGVKKRNNQKLTAFLNTFLSAITVMDWDYQVAEVYGQL
RAEMEREGKIMGVQDQMIGAHALATECVLVSSDKAFQFIPNLVLENWWK
Download Length: 390 bp
>T4914 NC_010278:c722396-722007 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA
ATGTATATGCTAGATACTAATACGGTGAGTTATTTCTTTAGGCAAGATCCTACTGTGGTTAAGAAACTACAACAACTTAA
TCCGGAATTGATTTGTATTTCATCGGTCACGGCTGCAGAGTTGTTTTATGGCGTAAAAAAACGTAATAATCAGAAACTTA
CTGCATTTTTAAATACTTTTTTATCTGCGATTACGGTGATGGATTGGGATTATCAAGTGGCGGAAGTTTATGGTCAGCTG
CGAGCAGAGATGGAACGAGAAGGCAAAATCATGGGCGTTCAAGATCAAATGATTGGAGCTCATGCCCTAGCAACAGAATG
TGTTCTTGTTTCCAGCGATAAAGCCTTTCAATTTATCCCTAATCTCGTATTAGAAAATTGGTGGAAGTAA
Antitoxin
Download Length: 87 a.a. Molecular weight: 10289.63 Da Isoelectric Point: 5.1867
>AT4914 WP_005604117.1 NC_010278:c722657-722397 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF
MEKVARLFRNGRNQAVRLPVEFEFNAKQVYVFKEANGDITLSTRSRNQQNWQKVFELLPMLECDEQFLSEQERKQEMATR
DPFEGF
Download Length: 261 bp
>AT4914 NC_010278:c722657-722397 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA
ATGGAAAAGGTCGCACGTTTATTTAGAAACGGACGTAATCAAGCCGTACGATTACCGGTTGAATTTGAGTTTAATGCGAA
GCAAGTTTATGTGTTTAAAGAAGCAAACGGCGATATTACGCTTTCGACACGTTCTCGCAATCAGCAAAATTGGCAGAAAG
TCTTTGAGTTATTACCAATGCTTGAATGTGATGAACAGTTTTTATCGGAACAAGAACGCAAGCAAGAAATGGCAACTCGA
GATCCGTTCGAGGGCTTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T4922 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
37.5 |
99.225 |
0.372 |
| T439 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
37.5 |
99.225 |
0.372 |
| T10054 | Bosea sp. PAMC 26642 strain PAMC26642 |
36.434 |
100 |
0.364 |
| T1070 | Haemophilus influenzae Rd KW20 |
35.938 |
99.225 |
0.357 |
| T877 | Sinorhizobium meliloti 1021 |
34.351 |
100 |
0.349 |
| T652 | Leptospira interrogans serovar Lai str. 56601 |
34.351 |
100 |
0.349 |
| T962 | Synechocystis sp. PCC 6803 |
39.437 |
85.542 |
0.337 |
| T3858 | Dichelobacter nodosus VCS1703A |
33.858 |
98.45 |
0.333 |
| T3856 | Dichelobacter nodosus VCS1703A |
33.858 |
98.45 |
0.333 |
| T10197 | Klebsiella pneumoniae |
31.343 |
100 |
0.326 |
| T10034 | Thermus thermophilus HB27 |
30.37 |
100 |
0.318 |
| T6111 | uncultured bacterium |
29.412 |
100 |
0.31 |
| T843 | Bradyrhizobium diazoefficiens USDA 110 |
33.051 |
91.473 |
0.302 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10033 | Thermus thermophilus HB27 |
31.111 |
100 |
0.326 |
| AT3856 | Dichelobacter nodosus VCS1703A |
38.71 |
81.579 |
0.316 |
| AT3858 | Dichelobacter nodosus VCS1703A |
38.71 |
81.579 |
0.316 |
Multiple sequence alignment
References
(1) Chengkun Zheng et al. (2017) Identification of four type II toxin-antitoxin systems in Actinobacillus pleuropneumoniae. FEMS Microbiology Letters 364(12):10.1093/femsle/fnx126. [PubMed:28637172]