Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vagCD/-
Location 155487..156298 Replicon plasmid pRSB225
Accession NC_025100
Organism uncultured bacterium

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag pRSB225_204 Protein ID YP_009066159.1
Coordinates 155487..155888 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID -
Locus tag pRSB225_205 Protein ID YP_009066160.1
Coordinates 155900..156298 (-) Length 133 a.a.

Genomic Context

pRSB225_199pRSB225_200pRSB225_201pRSB225_206pRSB225_198pRSB225_202pRSB225_203pRSB225_204pRSB225_205pRSB225_207pRSB225_208
Location: 151889..152164 (276 bp)
Type: Others
Protein ID: YP_009066154.1
Location: 152083..152586 (504 bp)
Type: Others
Protein ID: YP_009066155.1
Location: 152620..152784 (165 bp)
Type: Others
Protein ID: YP_009066156.1
Location: 156481..160305 (3825 bp)
Type: Others
Protein ID: YP_009066161.1
Location: 150619..151533 (915 bp)
Type: Others
Protein ID: YP_009066153.1
Location: 152840..153862 (1023 bp)
Type: Others
Protein ID: YP_009066157.1
Location: 153847..155412 (1566 bp)
Type: Others
Protein ID: YP_009066158.1
Location: 155487..155888 (402 bp)
Type: Toxin
Protein ID: YP_009066159.1
Location: 155900..156298 (399 bp)
Type: Antitoxin
Protein ID: YP_009066160.1
Location: 160350..160751 (402 bp)
Type: Others
Protein ID: YP_009066162.1
Location: 160763..160993 (231 bp)
Type: Others
Protein ID: YP_009066163.1
ToxinAntitoxinOther genes
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Locus tag
Coordinates
Strand
Size (bp)
Protein ID
Product
Description
pRSB225_198150619..151533-915YP_009066153.1iron transport system periplasmic binding protein-
pRSB225_199151889..152164+276YP_009066154.1insertion element IS1 protein InsA-
pRSB225_200152083..152586+504YP_009066155.1insertion element protein InsB-
pRSB225_201152620..152784+165YP_009066156.1hypothetical protein-
pRSB225_202152840..153862-1023YP_009066157.1hypothetical protein-
pRSB225_203153847..155412-1566YP_009066158.1hypothetical protein-
pRSB225_204155487..155888-402YP_009066159.1virulence associated protein DToxin
pRSB225_205155900..156298-399YP_009066160.1virulence associated protein CAntitoxin
pRSB225_206156481..160305+3825YP_009066161.1hypothetical protein-
pRSB225_207160350..160751-402YP_009066162.1virulence associated protein D-
pRSB225_208160763..160993-231YP_009066163.1virulence associated protein C-

Associated MGEs

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MGE
detail
Similar
MGEs
Relative
position
MGE Type
Cargo ARG
Virulence gene
Coordinates
Length (bp)
-insideConjugative plasmidaph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / mph(A) / dfrA14 / catA1 / tet(B) / sitABCDiutA / iucD / iucC / iucB / iucA1..164550164550
-flankIS/TnsitABCD-148084..1525864502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

SpoVT-AbrB domain

(63-108)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14614.92 Da        Isoelectric Point: 6.4752

>T6111 YP_009066159.1 NC_025100:c155888-155487 [uncultured bacterium]
MLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDAT
TEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 402 bp

>T6111 NC_025100:c155888-155487 [uncultured bacterium]
ATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCTGGAGCAGTCGGTGCT
GCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTCCGAAGGCCTCGCCAC
GCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCCGCGGTGGATGCGACC
ACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGCCGGACACGCCATCGC
TGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGGAAGACTGGGTAAGAT
AA

Antitoxin


Download         Length: 133 a.a.        Molecular weight: 15324.70 Da        Isoelectric Point: 6.1313

>AT6111 YP_009066160.1 NC_025100:c156298-155900 [uncultured bacterium]
MPDICIFMVYAYPHHLHMLNLYICLTSAYTKHMHTPGAMNIIPDMHHISTLTRERIMRTVSIFKNGNNRAIRLPRDLDFE
GVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 399 bp

>AT6111 NC_025100:c156298-155900 [uncultured bacterium]
ATGCCTGACATCTGCATATTCATGGTATACGCATATCCTCACCATCTGCATATGCTGAATCTATACATATGCCTAACATC
TGCCTATACTAAACATATGCACACACCCGGTGCAATGAACATCATTCCGGATATGCATCACATATCCACACTTACCCGGG
AGAGAATCATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAG
GGGGTGAGCGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCT
GGAGCTAGAAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
T6112uncultured bacterium

93.893

98.496

0.925

T10197Klebsiella pneumoniae

89.474

100

0.895

T439Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

41.985

99.242

0.417

T10054Bosea sp. PAMC 26642 strain PAMC26642

39.394

100

0.394

T4922Actinobacillus pleuropneumoniae serovar 3 str. JL03

38.636

100

0.386

T3858Dichelobacter nodosus VCS1703A

38.636

99.248

0.383

T3856Dichelobacter nodosus VCS1703A

38.636

99.248

0.383

T1070Haemophilus influenzae Rd KW20

36.364

100

0.364

T877Sinorhizobium meliloti 1021

36.364

99.248

0.361

T10129Haemophilus influenzae strain 69P2H1 N69P2H1_45_2

33.835

100

0.338
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1025
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Multiple sequence alignment    


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Protein
Organism
Identities (%)
Coverage (%)
Ha-value
AT6112uncultured bacterium

90.789

100

0.908

AT10197Klebsiella pneumoniae

91.781

96.053

0.882

AT652Leptospira interrogans serovar Lai str. 56601

36.986

96.053

0.355

AT439Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

36.232

92

0.333

AT3858Dichelobacter nodosus VCS1703A

37.879

86.842

0.329

AT3856Dichelobacter nodosus VCS1703A

37.879

86.842

0.329

AT4922Actinobacillus pleuropneumoniae serovar 3 str. JL03

37.5

84.211

0.316

AT1070Haemophilus influenzae Rd KW20

35.821

87.013

0.312

AT10033Thermus thermophilus HB27

29.078

100

0.308

AT962Synechocystis sp. PCC 6803

34.848

88

0.307
Showing 1 to 10 of 10 rows

Multiple sequence alignment    




Structures

Toxin

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Source
ID
Structure
No matching records found

Antitoxin

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Source
ID
Structure
No matching records found

References

(1) Daniel Wibberg et al. (2013) The IncF plasmid pRSB225 isolated from a municipal wastewater treatment plant's on-site preflooder combining antibiotic resistance and putative virulence functions is highly related to virulence plasmids identified in pathogenic E. coli isolates. Plasmid 69(2):127-37. [PubMed:23212116]