Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/AbrB-PIN
Location 351138..351775 Replicon chromosome
Accession NC_000907
Organism Haemophilus influenzae Rd KW20

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag HI_RS01715 Protein ID WP_010868975.1
Coordinates 351371..351775 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID E4QWH3
Locus tag HI_RS01710 Protein ID WP_005649046.1
Coordinates 351138..351374 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI_RS01685 (HI0315) 346365..347105 - 741 WP_005649034.1 YebC/PmpR family DNA-binding transcriptional regulator -
HI_RS01690 (HI0316) 347265..347741 - 477 WP_005694349.1 dihydroneopterin triphosphate diphosphatase -
HI_RS01695 (HI0317) 347763..349529 - 1767 WP_005694347.1 aspartate--tRNA ligase -
HI_RS01700 (HI0318) 349748..350266 + 519 WP_005649040.1 isoprenylcysteine carboxyl methyltransferase family protein -
HI_RS01705 (HI0319) 350319..351044 + 726 WP_005694346.1 carboxy-S-adenosyl-L-methionine synthase CmoA -
HI_RS01710 (HI0321) 351138..351374 + 237 WP_005649046.1 antitoxin Antitoxin
HI_RS01715 (HI0322) 351371..351775 + 405 WP_010868975.1 type II toxin-antitoxin system VapC family toxin Toxin
HI_RS01720 (HI0323) 351842..352249 + 408 WP_005688980.1 lactoylglutathione lyase -
HI_RS01725 (HI0324) 352323..353012 + 690 WP_005694345.1 ribonuclease T -
HI_RS01730 (HI0325) 353326..354678 + 1353 WP_005694344.1 Na+/H+ antiporter family protein -
HI_RS01735 (HI0326) 354711..355297 + 587 Protein_318 primosomal replication protein N -
HI_RS01745 (HI0328) 355648..356214 - 567 WP_005544066.1 elongation factor P -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-124)

Antitoxin

SpoVT-AbrB domain

(6-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15726.31 Da        Isoelectric Point: 8.9777

>T1066 WP_010868975.1 NC_000907:351371-351775 [Haemophilus influenzae Rd KW20]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWFACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Download         Length: 405 bp

>T1066 NC_000907:351371-351775 [Haemophilus influenzae Rd KW20]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGTTCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9037.19 Da        Isoelectric Point: 4.2342

>AT1066 WP_005649046.1 NC_000907:351138-351374 [Haemophilus influenzae Rd KW20]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Download         Length: 237 bp

>AT1066 NC_000907:351138-351374 [Haemophilus influenzae Rd KW20]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10129 Haemophilus influenzae strain 69P2H1 N69P2H1_45_2

99.254

100

0.993
T4922 Actinobacillus pleuropneumoniae serovar 3 str. JL03

40.152

100

0.402
T1070 Haemophilus influenzae Rd KW20

38.931

99.242

0.386
T439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

37.692

98.485

0.371
T652 Leptospira interrogans serovar Lai str. 56601

35.036

100

0.364
T10054 Bosea sp. PAMC 26642 strain PAMC26642

35.606

100

0.356
T10197 Klebsiella pneumoniae

35.075

100

0.351
T3856 Dichelobacter nodosus VCS1703A

35.659

96.992

0.346
T3858 Dichelobacter nodosus VCS1703A

35.659

96.992

0.346
T6111 uncultured bacterium

33.083

100

0.331
T6112 uncultured bacterium

32.576

99.248

0.323
T877 Sinorhizobium meliloti 1021

32.308

97.015

0.313
T962 Synechocystis sp. PCC 6803

41.935

74.699

0.313

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10129 Haemophilus influenzae strain 69P2H1 N69P2H1_45_2

100

100

1
AT652 Leptospira interrogans serovar Lai str. 56601

36.842

100

0.368
AT439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

32.5

100

0.347
AT3856 Dichelobacter nodosus VCS1703A

43.86

75

0.329
AT3858 Dichelobacter nodosus VCS1703A

43.86

75

0.329
AT137 Deinococcus radiodurans R1

30.38

100

0.308

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB E4QWH3

References

(1) Lauren R Walling et al. (2018) Homologous VapC Toxins Inhibit Translation and Cell Growth by Sequence-Specific Cleavage of tRNAfMet. Journal of Bacteriology 200(3):e00582-17. [PubMed:29109187] experimental

(2) Dayle A Daines et al. (2007) VapC-1 of nontypeable Haemophilus influenzae is a ribonuclease. Journal of Bacteriology 189(14):5041-8. [PubMed:17496075] experimental

(3) Lauren R Walling et al. (2016) Structural Determinants for Antitoxin Identity and Insulation of Cross Talk between Homologous Toxin-Antitoxin Systems. Journal of Bacteriology 198(24):3287-3295. [PubMed:27672196] experimental


experimental experimental literature