4922

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/AbrB-PIN
Location 1929188..1929816 Replicon chromosome
Accession NC_010278
Organism Actinobacillus pleuropneumoniae serovar 3 str. JL03

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag APJL_1767 Protein ID WP_012263372.1
Coordinates 1929418..1929816 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID B0BSF9
Locus tag APJL_1766 Protein ID WP_005616131.1
Coordinates 1929188..1929418 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
APJL_RS08895 (APJL_1764) 1926587..1928002 + 1416 WP_005605761.1 AAA family ATPase -
APJL_RS08900 (APJL_1765) 1927995..1928567 + 573 WP_012263371.1 hypothetical protein -
APJL_RS10920 1928940..1929080 - 141 WP_005602535.1 hypothetical protein -
APJL_RS08905 (APJL_1766) 1929188..1929418 + 231 WP_005616131.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
APJL_RS08910 (APJL_1767) 1929418..1929816 + 399 WP_012263372.1 tRNA(fMet)-specific endonuclease VapC Toxin
APJL_RS08915 (APJL_1768) 1929952..1930368 - 417 WP_012263373.1 DUF417 family protein -
APJL_RS08920 (APJL_1769) 1930614..1931264 + 651 WP_012263374.1 DUF2202 domain-containing protein -
APJL_RS08925 (APJL_1770) 1931337..1931996 - 660 WP_005605767.1 hypothetical protein -
APJL_RS08930 (APJL_1771) 1932310..1933632 - 1323 WP_012263375.1 HslU--HslV peptidase ATPase subunit -
APJL_RS08935 (APJL_1772) 1933898..1934419 - 522 WP_005618211.1 ATP-dependent protease subunit HslV -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1913969..1931996 18027


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

SpoVT-AbrB domain

(6-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15077.34 Da        Isoelectric Point: 7.4195

>T4922 WP_012263372.1 NC_010278:1929418-1929816 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MLTYMLDTNIAIYVVKRRPIEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLREFERVEGLRLDNWV

Download         Length: 399 bp

>T4922 NC_010278:1929418-1929816 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGCTAACTTATATGCTTGATACTAACATTGCGATTTATGTGGTTAAACGCAGACCTATTGAAGTGCTGGATAAGTTTAA
TTTAAATTCTACCCGTCTTTGTGTAAGTAGTATCACTGCAGCGGAGCTGTATTATGGTGCAGAGAAAAGCCAGTTTCCGG
AACGAAATATGGCAGTAATTGAGGATTTTCTTTCTCGTTTAACGATTCTTGATTATACGCATAAGGCAGCTACTCACTTT
GGCAATATCAAAGCACATTTATCTAAGCAAGGTAAGATCATTGGCGAGAACGATATTCATATTGCCGCACATGCAAGAAG
TGAAGGACTCGTATTAGTGACCAATAACTTGCGTGAATTTGAACGTGTGGAAGGATTGCGATTAGATAATTGGGTATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8595.68 Da        Isoelectric Point: 4.5358

>AT4922 WP_005616131.1 NC_010278:1929188-1929418 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLGSATVSDDFMTEREISTQAEREEL

Download         Length: 231 bp

>AT4922 NC_010278:1929188-1929418 [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGGAAGCATCAGTATTTATGACAAATCGCAGTCAAGCGGTACGATTGCCGGCAGAAGTACGTTTTGCAAGTGATGTAAA
AAAACTTTCTGTAAGAGTGGTAGGTAATGAACGTATTTTGGCACCGATTAACCAAAGTTGGGATAGCTTCTTTCTGGGGT
CGGCAACGGTAAGCGATGATTTCATGACAGAGCGTGAAATCAGCACTCAAGCCGAACGTGAGGAACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1070 Haemophilus influenzae Rd KW20

78.03

100

0.78
T439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

55.303

100

0.553
T10054 Bosea sp. PAMC 26642 strain PAMC26642

48.485

100

0.485
T3858 Dichelobacter nodosus VCS1703A

44.186

97.727

0.432
T3856 Dichelobacter nodosus VCS1703A

44.186

97.727

0.432
T10129 Haemophilus influenzae strain 69P2H1 N69P2H1_45_2

40.909

100

0.409
T1066 Haemophilus influenzae Rd KW20

40.152

100

0.402
T652 Leptospira interrogans serovar Lai str. 56601

40.769

98.485

0.402
T6112 uncultured bacterium

39.695

99.242

0.394
T10197 Klebsiella pneumoniae

38.806

100

0.394
T6111 uncultured bacterium

38.636

100

0.386
T962 Synechocystis sp. PCC 6803

44.444

86.747

0.386
T877 Sinorhizobium meliloti 1021

37.594

100

0.379
T4914 Actinobacillus pleuropneumoniae serovar 3 str. JL03

37.5

99.225

0.372

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT1070 Haemophilus influenzae Rd KW20

75

100

0.75
AT439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

45.946

98.667

0.453
AT652 Leptospira interrogans serovar Lai str. 56601

42.667

98.684

0.421
AT10054 Bosea sp. PAMC 26642 strain PAMC26642

39.189

97.368

0.382
AT3858 Dichelobacter nodosus VCS1703A

37.838

97.368

0.368
AT3856 Dichelobacter nodosus VCS1703A

37.838

97.368

0.368
AT6112 uncultured bacterium

37.5

84.211

0.316
AT6111 uncultured bacterium

37.5

84.211

0.316
AT10033 Thermus thermophilus HB27

29.71

100

0.311

Multiple sequence alignment    




Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB B0BSF9

References

(1) Chengkun Zheng et al. (2017) Identification of four type II toxin-antitoxin systems in Actinobacillus pleuropneumoniae. FEMS Microbiology Letters 364(12):10.1093/femsle/fnx126. [PubMed:28637172] experimental


experimental experimental literature