10197

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vagCD/-
Location 57017..57660 Replicon plasmid pKPS30
Accession NC_023314
Organism Klebsiella pneumoniae

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag HTC19_RS00330 Protein ID WP_000754566.1
Coordinates 57017..57433 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HTC19_RS00335 Protein ID WP_001261276.1
Coordinates 57430..57660 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTC19_RS00305 (IPF_197) 52419..53123 - 705 WP_001067834.1 IS6-like element IS26 family transposase -
HTC19_RS00310 (IPF_195) 53191..53712 + 522 Protein_61 transposase -
HTC19_RS00315 53875..53958 - 84 Protein_62 SOS response-associated peptidase -
HTC19_RS00320 (IPF_190) 54599..55675 - 1077 WP_000227969.1 IS110 family transposase -
HTC19_RS00325 55957..56813 - 857 Protein_64 IS3-like element ISEc15 family transposase -
HTC19_RS00330 (IPF_184) 57017..57433 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
HTC19_RS00335 (IPF_181) 57430..57660 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HTC19_RS00340 (IPF_179) 58234..58584 + 351 WP_000493378.1 hypothetical protein -
HTC19_RS00345 (IPF_178) 58635..59378 + 744 WP_000129823.1 hypothetical protein -
HTC19_RS00350 (IPF_175) 59375..60151 + 777 WP_000015958.1 site-specific integrase -
HTC19_RS00355 (IPF_173) 60209..60466 - 258 WP_000764642.1 hypothetical protein -
HTC19_RS00360 60595..60699 - 105 WP_032409716.1 hypothetical protein -
HTC19_RS00365 60975..61178 - 204 WP_159373846.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Mobilizable plasmid tet(A) / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / qnrB4 / blaDHA-1 / mph(A) / aph(3')-Ia - 1..61228 61228


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T10197 WP_000754566.1 NC_023314:c57433-57017 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T10197 NC_023314:c57433-57017 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT10197 WP_001261276.1 NC_023314:c57660-57430 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT10197 NC_023314:c57660-57430 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT6111 uncultured bacterium

91.781

96.053

0.882
AT6112 uncultured bacterium

83.784

97.368

0.816
AT10054 Bosea sp. PAMC 26642 strain PAMC26642

33.766

100

0.342
AT652 Leptospira interrogans serovar Lai str. 56601

35.616

96.053

0.342
AT439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

37.313

89.333

0.333
AT3858 Dichelobacter nodosus VCS1703A

36.364

86.842

0.316
AT3856 Dichelobacter nodosus VCS1703A

36.364

86.842

0.316
AT1070 Haemophilus influenzae Rd KW20

35.821

88.158

0.316
AT962 Synechocystis sp. PCC 6803

34.848

88

0.307

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References

(1) Marion Duprilot et al. (2017) Diversity and functionality of plasmid-borne VagCD toxin-antitoxin systems of Klebsiella pneumoniae. The Journal of Antimicrobial Chemotherapy 72(5):1320-1326. [PubMed:28119479] experimental


experimental experimental literature