Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20929..21572 Replicon plasmid pMBR_DHA-1_1CO
Accession NZ_CP049718
Organism Klebsiella pneumoniae strain 11KM256

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag G7Z27_RS27655 Protein ID WP_000754566.1
Coordinates 21156..21572 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag G7Z27_RS27650 Protein ID WP_001261276.1
Coordinates 20929..21159 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G7Z27_RS27615 16048..16317 + 270 WP_000339857.1 hypothetical protein -
G7Z27_RS27620 16494..17360 - 867 WP_004118283.1 replication initiation protein -
G7Z27_RS27625 17890..17994 + 105 WP_032409716.1 hypothetical protein -
G7Z27_RS27630 18123..18380 + 258 WP_000764642.1 hypothetical protein -
G7Z27_RS27635 18438..19214 - 777 WP_000015958.1 site-specific integrase -
G7Z27_RS27640 19211..19954 - 744 WP_000129823.1 hypothetical protein -
G7Z27_RS27645 20005..20355 - 351 WP_000493378.1 hypothetical protein -
G7Z27_RS27650 20929..21159 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G7Z27_RS27655 21156..21572 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
G7Z27_RS27660 21776..22632 + 857 Protein_23 IS3-like element ISEc15 family transposase -
G7Z27_RS27665 22914..23990 + 1077 WP_000227969.1 IS110 family transposase -
G7Z27_RS27670 24631..24714 + 84 Protein_25 SOS response-associated peptidase -
G7Z27_RS27675 24877..25401 - 525 Protein_26 Tn3 family transposase -
G7Z27_RS27680 25466..26170 + 705 WP_001067834.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Mobilizable plasmid aph(3')-Ia / mph(A) / sul1 / blaDHA-1 / qnrB4 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..55778 55778
- inside Integron - - 148..55679 55531


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T151454 WP_000754566.1 NZ_CP049718:21156-21572 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T151454 NZ_CP065515:c2527700-2527434 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAAATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT151454 WP_001261276.1 NZ_CP049718:20929-21159 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT151454 NZ_CP065515:c2527951-2527700 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References