Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 27494..28137 Replicon plasmid unnamed3
Accession NZ_CP061955
Organism Klebsiella pneumoniae strain IR5654

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag H4I75_RS27570 Protein ID WP_000754566.1
Coordinates 27494..27910 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag H4I75_RS27575 Protein ID WP_001261276.1
Coordinates 27907..28137 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4I75_RS27545 22896..23600 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
H4I75_RS27550 23665..24189 + 525 Protein_26 Tn3 family transposase -
H4I75_RS27555 24352..24435 - 84 Protein_27 SOS response-associated peptidase -
H4I75_RS27560 25076..26152 - 1077 WP_000227969.1 IS110 family transposase -
H4I75_RS27565 26434..27290 - 857 Protein_29 IS3-like element ISEc15 family transposase -
H4I75_RS27570 27494..27910 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
H4I75_RS27575 27907..28137 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H4I75_RS27580 28711..29061 + 351 WP_000493378.1 hypothetical protein -
H4I75_RS27585 29112..29855 + 744 WP_000129823.1 hypothetical protein -
H4I75_RS27590 29852..30628 + 777 WP_000015958.1 site-specific integrase -
H4I75_RS27595 30686..30943 - 258 WP_000764642.1 hypothetical protein -
H4I75_RS27600 31072..31176 - 105 WP_032409716.1 hypothetical protein -
H4I75_RS27605 31706..32572 + 867 WP_004118283.1 replication initiation protein -
H4I75_RS27610 32749..33018 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Mobilizable plasmid aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / qnrB4 / aph(3')-Ia - 1..44557 44557


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T173246 WP_000754566.1 NZ_CP061955:c27910-27494 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T173246 NZ_CP080121:2508416-2508523 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT173246 WP_001261276.1 NZ_CP061955:c28137-27907 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT173246 NZ_CP080121:c2508369-2508302 [Escherichia coli]
TGTCTGGTTTCAAGATTAGGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References