3856

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/AbrB-PIN
Location 296317..296951 Replicon chromosome
Accession NC_009446
Organism Dichelobacter nodosus VCS1703A

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag DNO_RS01345 Protein ID WP_119185609.1
Coordinates 296550..296951 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q46558
Locus tag DNO_RS01340 Protein ID WP_012030622.1
Coordinates 296317..296547 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNO_RS01300 (DNO_0267) 292206..292553 - 348 WP_012030613.1 hypothetical protein -
DNO_RS01305 (DNO_0269) 292557..292778 - 222 WP_012030615.1 hypothetical protein -
DNO_RS01310 (DNO_0271) 293128..293475 + 348 WP_012030616.1 HigA family addiction module antidote protein -
DNO_RS01315 (DNO_0270) 293468..293647 - 180 WP_041729402.1 hypothetical protein -
DNO_RS01320 (DNO_0272) 293791..294117 - 327 WP_012030618.1 HigA family addiction module antidote protein -
DNO_RS01325 (DNO_0273) 294127..294402 - 276 WP_012030619.1 type II toxin-antitoxin system RelE/ParE family toxin -
DNO_RS01330 (DNO_0274) 295382..295660 + 279 WP_041729404.1 type II toxin-antitoxin system RelE/ParE family toxin -
DNO_RS01335 (DNO_0275) 295673..295981 + 309 WP_012030621.1 HigA family addiction module antidote protein -
DNO_RS01340 (DNO_0276) 296317..296547 + 231 WP_012030622.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
DNO_RS01345 (DNO_0277) 296550..296951 + 402 WP_119185609.1 type II toxin-antitoxin system VapC family toxin Toxin
DNO_RS01350 (DNO_0278) 297073..297354 - 282 WP_012030611.1 virulence protein -
DNO_RS01355 (DNO_0279) 297992..299305 - 1314 WP_012030624.1 virulence-associated E family protein -
DNO_RS01360 (DNO_0280) 299401..299607 - 207 WP_012030625.1 hypothetical protein -
DNO_RS01365 (DNO_0282) 299986..301191 - 1206 WP_012030627.1 tyrosine-type recombinase/integrase -
DNO_RS01375 (DNO_0284) 301542..301736 - 195 WP_012030628.1 carbon storage regulator CsrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 282708..307332 24624


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-124)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14910.39 Da        Isoelectric Point: 7.8485

>T3856 WP_119185609.1 NC_009446:296550-296951 [Dichelobacter nodosus VCS1703A]
IKLMLDTNICIYIINRKPPYIAEKFSRYEIGDIGISNITACELAFGVEKSGSAKNKTALTKFLAPLSILPFDKQAIWHYA
RIRQSLQNRGTPIGALDMLIASHALALDITLVTNNTKEFERVDGLMLDNWVID

Download         Length: 402 bp

>T3856 NC_009446:296550-296951 [Dichelobacter nodosus VCS1703A]
ATCAAATTAATGCTTGATACCAATATCTGCATTTATATCATCAATCGAAAACCGCCATATATTGCCGAAAAATTTTCGCG
ATATGAAATAGGCGATATTGGTATATCTAACATTACCGCTTGTGAGCTTGCGTTTGGCGTAGAAAAATCAGGTTCAGCAA
AAAACAAAACCGCATTAACAAAATTTTTGGCACCACTATCAATATTGCCTTTTGATAAACAAGCTATTTGGCATTACGCA
CGAATCCGGCAATCACTGCAAAACCGCGGTACCCCTATCGGGGCTTTAGATATGCTTATCGCTAGCCATGCATTGGCGCT
TGATATCACGTTGGTAACAAATAATACCAAAGAATTTGAGCGCGTTGATGGGTTGATGTTAGATAATTGGGTGATAGATT
GA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8899.23 Da        Isoelectric Point: 5.0959

>AT3856 WP_012030622.1 NC_009446:296317-296547 [Dichelobacter nodosus VCS1703A]
MKVAKVFTTGRSQAVRIPKAFQFDTKEVIIQRFGNGILLIPKNSDWQGFLEALNEFEPDFKIEREPQIEQVREDFL

Download         Length: 231 bp

>AT3856 NC_009446:296317-296547 [Dichelobacter nodosus VCS1703A]
ATGAAAGTAGCCAAAGTCTTTACTACGGGGCGCTCGCAAGCGGTTCGCATTCCTAAGGCGTTCCAATTTGATACAAAAGA
GGTAATTATTCAGCGTTTCGGCAACGGAATTTTATTAATCCCTAAAAATTCGGATTGGCAAGGTTTTCTGGAAGCACTCA
ATGAGTTTGAGCCTGATTTTAAAATTGAGCGTGAGCCGCAAATAGAACAAGTGCGGGAAGACTTTTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T3858 Dichelobacter nodosus VCS1703A

100

100

1
T962 Synechocystis sp. PCC 6803

48.193

100

0.482
T10054 Bosea sp. PAMC 26642 strain PAMC26642

44.961

97.727

0.439
T4922 Actinobacillus pleuropneumoniae serovar 3 str. JL03

44.186

97.727

0.432
T652 Leptospira interrogans serovar Lai str. 56601

42.188

96.97

0.409
T1070 Haemophilus influenzae Rd KW20

40.909

100

0.409
T439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

41.221

99.242

0.409
T877 Sinorhizobium meliloti 1021

42.188

96.241

0.406
T10197 Klebsiella pneumoniae

40.152

99.248

0.399
T6112 uncultured bacterium

38.931

98.496

0.383
T6111 uncultured bacterium

38.636

99.248

0.383
T10129 Haemophilus influenzae strain 69P2H1 N69P2H1_45_2

36.434

96.992

0.353
T1066 Haemophilus influenzae Rd KW20

35.659

96.992

0.346
T4914 Actinobacillus pleuropneumoniae serovar 3 str. JL03

33.858

98.45

0.333

Multiple sequence alignment    



Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A5EW96

References

(1) B F Cheetham et al. (1995) Identification of a gene encoding a bacteriophage-related integrase in a vap region of the Dichelobacter nodosus genome. Gene 162(1):53-8. [PubMed:7557417] experimental


experimental experimental literature