10129

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/-
Location 23205..23842 Replicon chromosome
Accession NZ_MZIN01000044
Organism Haemophilus influenzae strain 69P2H1 N69P2H1_45_2

Toxin (Protein)


Gene name VapC1 Uniprot ID E4QWH2
Locus tag BV136_RS07045 Protein ID WP_005649049.1
Coordinates 23438..23842 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name VapB1 Uniprot ID E4QWH3
Locus tag BV136_RS07040 Protein ID WP_005649046.1
Coordinates 23205..23441 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BV136_RS07015 (BV136_01361) 18584..19324 - 741 WP_005649034.1 YebC/PmpR family DNA-binding transcriptional regulator -
BV136_RS07020 (BV136_01362) 19336..19809 - 474 WP_011271985.1 dihydroneopterin triphosphate diphosphatase -
BV136_RS07025 (BV136_01363) 19831..21597 - 1767 WP_011271986.1 aspartate--tRNA ligase -
BV136_RS07030 (BV136_01364) 21816..22334 + 519 WP_005649040.1 isoprenylcysteine carboxyl methyltransferase family protein -
BV136_RS07035 (BV136_01365) 22387..23112 + 726 WP_105892704.1 carboxy-S-adenosyl-L-methionine synthase CmoA -
BV136_RS07040 (BV136_01366) 23205..23441 + 237 WP_005649046.1 antitoxin Antitoxin
BV136_RS07045 (BV136_01367) 23438..23842 + 405 WP_005649049.1 type II toxin-antitoxin system VapC family toxin Toxin
BV136_RS07050 (BV136_01368) 23909..24316 + 408 WP_005688980.1 lactoylglutathione lyase -
BV136_RS07055 (BV136_01369) 24390..25079 + 690 WP_011271988.1 ribonuclease T -
BV136_RS07060 (BV136_01370) 25393..26745 + 1353 WP_005694344.1 Na+/H+ antiporter family protein -
BV136_RS07065 (BV136_01371) 26778..27365 + 588 WP_005654063.1 primosomal replication protein PriC -
BV136_RS07075 (BV136_01373) 27716..28282 - 567 WP_005544066.1 elongation factor P -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-124)

Antitoxin

SpoVT-AbrB domain

(6-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15692.29 Da        Isoelectric Point: 8.9777

>T10129 WP_005649049.1 NZ_MZIN01000044:23438-23842 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Download         Length: 405 bp

>T10129 NZ_MZIN01000044:23438-23842 [Haemophilus influenzae]
ATGATTTATATGTTAGACACCAATATCATTATTTATTTAATGAAAAATCGCCCCAAAATTATTGCCGAACGAGTATCACA
ATTATTGCCTAATGATCGCTTAGTTATGAGCTTTATTACTTATGCTGAACTTATTAAAGGCGCCTTTGGTAGTCAAAATT
ATGAGCAATCAATACGAGCAATAGAATTACTTACTGAACGAGTGAATGTACTATATCCCAATGAACAAATCTGTTTACAT
TATGGCAAATGGGCAAATACACTCAAAAAACAAGGGCGACCTATCGGAAATAATGATCTATGGATCGCTTGTCACGCATT
GAGTTTAAATGCCGTTCTTATTACACATAATGTAAAAGAATTTCAGCGAATTACAGATCTTCAGTGGCAAGATTGGACAA
AATAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9037.19 Da        Isoelectric Point: 4.2342

>AT10129 WP_005649046.1 NZ_MZIN01000044:23205-23441 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Download         Length: 237 bp

>AT10129 NZ_MZIN01000044:23205-23441 [Haemophilus influenzae]
ATGCTTACTAAAGTGTTTCAAAGTGGTAACAGCCAAGCTGTTCGGATCCCGATGGACTTTCGTTTTGACGTCGATACCGT
AGAAATTTTCCGAAAGGAAAATGGGGATGTGGTATTACGCCCAGTTTCTAAAAAAACAGATGATTTTCTTGCGTTATTTG
AAGGATTTGATGAGACCTTTATTCAAGCACTTGAAGCGCGTGATGATTTACCGCCTCAGGAGCGAGAAAATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T1066 Haemophilus influenzae Rd KW20

99.254

100

0.993
T4922 Actinobacillus pleuropneumoniae serovar 3 str. JL03

40.909

100

0.409
T1070 Haemophilus influenzae Rd KW20

39.695

99.242

0.394
T439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

38.462

98.485

0.379
T10054 Bosea sp. PAMC 26642 strain PAMC26642

36.364

100

0.364
T652 Leptospira interrogans serovar Lai str. 56601

35.036

100

0.364
T10197 Klebsiella pneumoniae

35.821

100

0.358
T3858 Dichelobacter nodosus VCS1703A

36.434

96.992

0.353
T3856 Dichelobacter nodosus VCS1703A

36.434

96.992

0.353
T6111 uncultured bacterium

33.835

100

0.338
T6112 uncultured bacterium

33.333

99.248

0.331
T877 Sinorhizobium meliloti 1021

33.077

97.015

0.321
T962 Synechocystis sp. PCC 6803

41.935

74.699

0.313

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT1066 Haemophilus influenzae Rd KW20

100

100

1
AT652 Leptospira interrogans serovar Lai str. 56601

36.842

100

0.368
AT439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

32.5

100

0.347
AT3856 Dichelobacter nodosus VCS1703A

43.86

75

0.329
AT3858 Dichelobacter nodosus VCS1703A

43.86

75

0.329
AT137 Deinococcus radiodurans R1

30.38

100

0.308

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB E4QWH2

Antitoxin

Source ID Structure
AlphaFold DB E4QWH3

References

(1) Ashley L Molinaro et al. (2019) Crystal Structure of VapBC-1 from Nontypeable Haemophilus influenzae and the Effect of PIN Domain Mutations on Survival during Infection. Journal of Bacteriology 201(12):e00026-19. [PubMed:30936373]