652

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/AbrB-PIN
Location 1007753..1008381 Replicon chromosome
Accession NC_004342
Organism Leptospira interrogans serovar Lai str. 56601

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag LA_RS04175 Protein ID WP_000281042.1
Coordinates 1007923..1008321 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag LA_RS04180 Protein ID WP_001192482.1
Coordinates 1008321..1008551 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA_RS04150 (LA0996) 1003215..1003982 + 768 WP_000636952.1 DUF1554 domain-containing protein -
LA_RS04155 (LA0997) 1004523..1005383 + 861 WP_000682233.1 hypothetical protein -
LA_RS04160 (LA0997a) 1005600..1006058 - 459 WP_001239004.1 GNAT family N-acetyltransferase -
LA_RS04165 (LA0999) 1006059..1007135 - 1077 WP_001170569.1 hypothetical protein -
LA_RS04170 (LA1000) 1007277..1007825 - 549 WP_000479564.1 hypothetical protein -
LA_RS04175 (LA1001) 1007923..1008321 - 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
LA_RS04180 (LA1002) 1008321..1008551 - 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LA_RS04185 (LA1003) 1008799..1009476 - 678 WP_000071998.1 hypothetical protein -
LA_RS04190 (LA1004) 1009708..1010397 - 690 WP_000590463.1 SH3 domain-containing protein -
LA_RS04195 (LA1005) 1010623..1011246 - 624 Protein_828 M20/M25/M40 family metallo-hydrolase -
LA_RS04200 (LA1006) 1011496..1012569 + 1074 WP_000445690.1 IS110-like element ISLin1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 1011496..1012569 1073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T652 WP_000281042.1 NC_004342:c1008321-1007923 [Leptospira interrogans serovar Lai str. 56601]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T652 NC_004342:c1008321-1007923 [Leptospira interrogans serovar Lai str. 56601]
ATGTATCTTTTGGATACCAATATATGCATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT652 WP_001192482.1 NC_004342:c1008551-1008321 [Leptospira interrogans serovar Lai str. 56601]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT652 NC_004342:c1008551-1008321 [Leptospira interrogans serovar Lai str. 56601]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTTCGACTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTTATCCAAAAAGTTGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGAGAGAGAACAGCCGAAATCTGATAAAAGAGAAAAGCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T962 Synechocystis sp. PCC 6803

46.053

91.566

0.422
T3856 Dichelobacter nodosus VCS1703A

42.188

96.97

0.409
T3858 Dichelobacter nodosus VCS1703A

42.188

96.97

0.409
T1070 Haemophilus influenzae Rd KW20

41.538

98.485

0.409
T4922 Actinobacillus pleuropneumoniae serovar 3 str. JL03

40.769

98.485

0.402
T10054 Bosea sp. PAMC 26642 strain PAMC26642

41.085

97.727

0.402
T439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

36.364

100

0.364
T1066 Haemophilus influenzae Rd KW20

35.036

100

0.364
T10129 Haemophilus influenzae strain 69P2H1 N69P2H1_45_2

35.036

100

0.364
T877 Sinorhizobium meliloti 1021

35.606

100

0.356
T4914 Actinobacillus pleuropneumoniae serovar 3 str. JL03

34.351

100

0.349
T10197 Klebsiella pneumoniae

30.597

100

0.311
T10138 Pseudomonas savastanoi

28.986

100

0.303
T6111 uncultured bacterium

29.851

100

0.303

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT3856 Dichelobacter nodosus VCS1703A

52.703

97.368

0.513
AT3858 Dichelobacter nodosus VCS1703A

52.703

97.368

0.513
AT962 Synechocystis sp. PCC 6803

45.333

100

0.453
AT10054 Bosea sp. PAMC 26642 strain PAMC26642

42.308

100

0.434
AT439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

42.105

100

0.427
AT4922 Actinobacillus pleuropneumoniae serovar 3 str. JL03

42.667

98.684

0.421
AT1070 Haemophilus influenzae Rd KW20

38.462

100

0.395
AT1066 Haemophilus influenzae Rd KW20

36.842

100

0.368
AT10129 Haemophilus influenzae strain 69P2H1 N69P2H1_45_2

36.842

100

0.368
AT6111 uncultured bacterium

36.986

96.053

0.355
AT663 Streptococcus mutans UA159

36.62

93.421

0.342
AT10197 Klebsiella pneumoniae

35.616

96.053

0.342
AT6112 uncultured bacterium

35.616

96.053

0.342

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References

(1) Yi Xuan Zhang et al. (2004) Characterization of a novel toxin-antitoxin module, VapBC, encoded by Leptospira interrogans chromosome. Cell Research 14(3):208-16. [PubMed:15225414] experimental

(2) Alexandre P Y Lopes et al. (2014) VapC from the leptospiral VapBC toxin-antitoxin module displays ribonuclease activity on the initiator tRNA. PloS One 9(7):e101678. [PubMed:25047537] experimental


experimental experimental literature