Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/AbrB-PIN |
| Location | 473953..474432 | Replicon | chromosome |
| Accession | NC_000911 | ||
| Organism | Synechocystis sp. PCC 6803 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | ssl2923 | Protein ID | WP_010871640.1 |
| Coordinates | 473953..474204 (-) | Length | 84 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | - |
| Locus tag | ssl2922 | Protein ID | WP_010871641.1 |
| Coordinates | 474205..474432 (-) | Length | 76 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| SGL_RS04105 (sll1507) | 469001..469546 | - | 546 | WP_010871636.1 | alpha/beta hydrolase | - |
| SGL_RS04110 (slr1603) | 469592..470914 | + | 1323 | WP_010871637.1 | hypothetical protein | - |
| SGL_RS04115 (slr1604) | 471080..472930 | + | 1851 | WP_010871638.1 | ATP-dependent zinc metalloprotease FtsH3 | - |
| SGL_RS04120 | 473088..473675 | + | 588 | Protein_446 | IS5 family transposase | - |
| SGL_RS04125 (ssl2923) | 473953..474204 | - | 252 | WP_010871640.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| SGL_RS04130 (ssl2922) | 474205..474432 | - | 228 | WP_010871641.1 | virulence factor | Antitoxin |
| SGL_RS04135 | 474511..474792 | - | 282 | WP_020861443.1 | type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family | - |
| SGL_RS04140 (ssl2920) | 474789..475007 | - | 219 | WP_010871642.1 | hypothetical protein | - |
| SGL_RS04145 (sll1505) | 475110..475445 | - | 336 | WP_010871643.1 | DUF86 domain-containing protein | - |
| SGL_RS04150 (sll1504) | 475435..475743 | - | 309 | WP_010871644.1 | nucleotidyltransferase family protein | - |
| SGL_RS04155 (sll1503) | 475873..477399 | - | 1527 | WP_010871645.1 | RNA-directed DNA polymerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| flank | IS/Tn | - | - | 473414..473662 | 248 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 84 a.a. Molecular weight: 9540.13 Da Isoelectric Point: 7.9952
>T962 WP_010871640.1 NC_000911:c474204-473953 [Synechocystis sp. PCC 6803]
MKYLLDTNICIYLIKKKPFKVLAKFQTLEISDIGISSITVAELEYGVSKSQQQSKNRDALMQFLMPLEIVEFNSGSGDRL
WQH
MKYLLDTNICIYLIKKKPFKVLAKFQTLEISDIGISSITVAELEYGVSKSQQQSKNRDALMQFLMPLEIVEFNSGSGDRL
WQH
Download Length: 252 bp
>T962 NC_000911:c474204-473953 [Synechocystis sp. PCC 6803]
ATGAAGTATTTACTAGATACGAATATTTGCATTTATCTCATTAAGAAGAAACCGTTTAAAGTCTTGGCAAAATTTCAAAC
CCTGGAGATATCAGACATTGGTATTTCATCCATTACCGTGGCGGAATTAGAATATGGTGTCAGTAAAAGTCAACAACAAT
CGAAAAATCGTGATGCCTTAATGCAATTCTTGATGCCGCTAGAAATCGTGGAATTTAATTCAGGAAGCGGCGATCGCCTA
TGGCAACATTAG
ATGAAGTATTTACTAGATACGAATATTTGCATTTATCTCATTAAGAAGAAACCGTTTAAAGTCTTGGCAAAATTTCAAAC
CCTGGAGATATCAGACATTGGTATTTCATCCATTACCGTGGCGGAATTAGAATATGGTGTCAGTAAAAGTCAACAACAAT
CGAAAAATCGTGATGCCTTAATGCAATTCTTGATGCCGCTAGAAATCGTGGAATTTAATTCAGGAAGCGGCGATCGCCTA
TGGCAACATTAG
Antitoxin
Download Length: 76 a.a. Molecular weight: 8538.69 Da Isoelectric Point: 4.2840
>AT962 WP_010871641.1 NC_000911:c474432-474205 [Synechocystis sp. PCC 6803]
MNTAQISTDGTHQIVILPENFTIAGSEVYIKKIGSTIILIAKNNPWQSLIESLDQFSDDFMKTREQPPLDIREEF
MNTAQISTDGTHQIVILPENFTIAGSEVYIKKIGSTIILIAKNNPWQSLIESLDQFSDDFMKTREQPPLDIREEF
Download Length: 228 bp
>AT962 NC_000911:c474432-474205 [Synechocystis sp. PCC 6803]
ATGAACACAGCTCAAATTAGCACCGATGGCACCCATCAAATCGTTATTTTACCAGAAAACTTTACGATAGCCGGAAGCGA
AGTTTACATTAAAAAAATTGGAAGTACAATTATTTTGATTGCTAAAAATAACCCTTGGCAATCCTTAATTGAAAGCTTAG
ATCAATTTTCAGATGATTTTATGAAAACTAGGGAACAGCCTCCCTTAGATATACGAGAAGAGTTCTAA
ATGAACACAGCTCAAATTAGCACCGATGGCACCCATCAAATCGTTATTTTACCAGAAAACTTTACGATAGCCGGAAGCGA
AGTTTACATTAAAAAAATTGGAAGTACAATTATTTTGATTGCTAAAAATAACCCTTGGCAATCCTTAATTGAAAGCTTAG
ATCAATTTTCAGATGATTTTATGAAAACTAGGGAACAGCCTCCCTTAGATATACGAGAAGAGTTCTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T3858 | Dichelobacter nodosus VCS1703A |
48.193 |
100 |
0.482 |
| T3856 | Dichelobacter nodosus VCS1703A |
48.193 |
100 |
0.482 |
| T652 | Leptospira interrogans serovar Lai str. 56601 |
46.053 |
91.566 |
0.422 |
| T4922 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
44.444 |
86.747 |
0.386 |
| T1070 | Haemophilus influenzae Rd KW20 |
43.056 |
86.747 |
0.373 |
| T439 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
39.189 |
89.157 |
0.349 |
| T4914 | Actinobacillus pleuropneumoniae serovar 3 str. JL03 |
39.437 |
85.542 |
0.337 |
| T10054 | Bosea sp. PAMC 26642 strain PAMC26642 |
38.356 |
87.952 |
0.337 |
| T10129 | Haemophilus influenzae strain 69P2H1 N69P2H1_45_2 |
41.935 |
74.699 |
0.313 |
| T1066 | Haemophilus influenzae Rd KW20 |
41.935 |
74.699 |
0.313 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT652 | Leptospira interrogans serovar Lai str. 56601 |
45.333 |
100 |
0.453 |
| AT3858 | Dichelobacter nodosus VCS1703A |
38.667 |
100 |
0.387 |
| AT3856 | Dichelobacter nodosus VCS1703A |
38.667 |
100 |
0.387 |
| AT10054 | Bosea sp. PAMC 26642 strain PAMC26642 |
32.895 |
100 |
0.333 |
| AT6111 | uncultured bacterium |
34.848 |
88 |
0.307 |
| AT10197 | Klebsiella pneumoniae |
34.848 |
88 |
0.307 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Stefan Kopfmann et al. (2016) Type II Toxin-Antitoxin Systems in the Unicellular Cyanobacterium Synechocystis sp. PCC 6803. Toxins 8(7):228. [PubMed:27455323]