Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/AbrB-PIN
Location 1008185..1008816 Replicon chromosome
Accession NC_000907
Organism Haemophilus influenzae Rd KW20

Toxin (Protein)


Gene name vapC Uniprot ID Q4QLW0
Locus tag HI0947 Protein ID WP_005651560.1
Coordinates 1008185..1008583 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q4QLV9
Locus tag HI0948 Protein ID WP_005648011.1
Coordinates 1008583..1008816 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI_RS05000 (HI0944) 1003367..1004485 + 1119 WP_005693291.1 bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD -
HI_RS05005 (HI0945) 1004486..1005508 + 1023 WP_010869093.1 outer membrane-stress sensor serine endopeptidase DegS -
HI_RS05010 (HI0946) 1005582..1006397 - 816 WP_005693293.1 DNA-formamidopyrimidine glycosylase -
HI_RS05015 (HI0946.1) 1006630..1008165 - 1536 WP_005693296.1 L-2,4-diaminobutyrate decarboxylase -
HI_RS05020 (HI0947) 1008185..1008583 - 399 WP_005651560.1 tRNA(fMet)-specific endonuclease VapC Toxin
HI_RS05025 (HI0948) 1008583..1008816 - 234 WP_005648011.1 type II toxin-antitoxin system antitoxin VapB2 Antitoxin
HI_RS05030 (HI0949) 1008963..1010327 - 1365 WP_005693298.1 diaminobutyrate--2-oxoglutarate transaminase -
HI_RS05035 (HI0950) 1010675..1010845 - 171 WP_005613503.1 50S ribosomal protein L33 -
HI_RS05040 (HI0951) 1010857..1011093 - 237 WP_005542826.1 50S ribosomal protein L28 -
HI_RS05045 (HI0952) 1011307..1011972 - 666 WP_010869094.1 DNA repair protein RadC -
HI_RS05050 (HI0953) 1012136..1013338 + 1203 WP_005693302.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

SpoVT-AbrB domain

(7-56)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15115.46 Da        Isoelectric Point: 8.0514

>T1070 WP_005651560.1 NC_000907:c1008583-1008185 [Haemophilus influenzae Rd KW20]
VLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSEYPERNIAVIEDFLSRLTILDYQPKHAAHF
GNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Download         Length: 399 bp

>T1070 NC_000907:c1008583-1008185 [Haemophilus influenzae Rd KW20]
GTGTTGAAATATATGCTTGATACTAACATTGTGATTTACGTGATTAAGCGTAGGCCATTGGAAATTCTTTCTCGCTTTAA
TCAAAATGCAGGAAAAATGTGCGTGAGTAGCATTACTGTGGCTGAACTCTATTATGGAGCTGAAAAAAGCGAATATCCCG
AAAGAAACATTGCGGTTATTGAAGATTTCTTATCACGCCTCACCATTTTAGATTACCAACCAAAGCATGCAGCTCATTTC
GGCAATATTAAAGCTGAACTCTCTAAACAAGGGAAATTGATTGGCGAAAATGATATTCATATAGCGGCTCATGCTCGTAG
TGAAGGGTTAATTCTGGTTAGTAACAATTTAAGAGAATTTGAGCGAGTTATAGCATTACGTACAGAAAATTGGGTGTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8880.98 Da        Isoelectric Point: 4.4013

>AT1070 WP_005648011.1 NC_000907:c1008816-1008583 [Haemophilus influenzae Rd KW20]
MIEASVFMTNRSQAVRLPAEVRFSEEIKKLSVRVSGSDRILSPLNQSWDSFFLNDQAVSDDFMNEREIAFQPEREAL

Download         Length: 234 bp

>AT1070 NC_000907:c1008816-1008583 [Haemophilus influenzae Rd KW20]
ATGATTGAAGCCTCTGTTTTTATGACTAATCGAAGTCAAGCTGTGCGTTTGCCCGCTGAAGTTCGCTTTTCTGAAGAAAT
CAAAAAATTATCGGTTCGTGTATCGGGCAGTGATCGGATTTTATCGCCTCTCAACCAATCTTGGGATAGTTTTTTCTTGA
ATGATCAAGCAGTTAGCGATGATTTTATGAATGAACGAGAAATTGCATTTCAACCAGAGCGTGAGGCTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
AT4922 Actinobacillus pleuropneumoniae serovar 3 str. JL03

75

100

0.75
AT439 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

39.474

100

0.4
AT652 Leptospira interrogans serovar Lai str. 56601

38.462

100

0.395
AT3856 Dichelobacter nodosus VCS1703A

36.111

94.737

0.342
AT3858 Dichelobacter nodosus VCS1703A

36.111

94.737

0.342
AT10054 Bosea sp. PAMC 26642 strain PAMC26642

41.935

80.519

0.338
AT6112 uncultured bacterium

37.313

88.158

0.329
AT10197 Klebsiella pneumoniae

35.821

88.158

0.316
AT6111 uncultured bacterium

35.821

87.013

0.312
AT10033 Thermus thermophilus HB27

29.71

100

0.311

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB Q4QLW0

Antitoxin

Source ID Structure
AlphaFold DB A0A806DGS0

References

(1) Lauren R Walling et al. (2016) Structural Determinants for Antitoxin Identity and Insulation of Cross Talk between Homologous Toxin-Antitoxin Systems. Journal of Bacteriology 198(24):3287-3295. [PubMed:27672196] experimental

(2) Lauren R Walling et al. (2018) Homologous VapC Toxins Inhibit Translation and Cell Growth by Sequence-Specific Cleavage of tRNAfMet. Journal of Bacteriology 200(3):e00582-17. [PubMed:29109187] experimental


experimental experimental literature